B-LCL-HROM01








General information
Description | The cell line was derived from the peripheral blood of a patient with CRC. |
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Organism | Human |
Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | TiBc HROM |
Characteristics
Morphology | Round cells |
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Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROM01 (Cytion catalog number 302079) |
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Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Viruses | Transformant: EBV |
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Handling
Culture Medium | RPMI 1640 |
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Medium supplements | 10% heat-inactivated FBS, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 |
Passaging solution | The utilization of a passaging solution is not necessary when passaging cells that are cultured in suspension. The appropriate procedure is to dilute the cells in accordance with the indicated guidelines. |
Subculturing | Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section. |
Handling of proliferating cultures | One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring. |
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HLA alleles |
A*: 01:01:01, 31:01:02
B*: 08:01:01, 14:02:01
C*: 07:01:01, 08:02:01
DRB1*: 03:01:01, 13:03:01
DQA1*: 05:01:01, 05:05:01
DQB1*: 02:01:01, 03:01:01
DPB1*: 01:01:01, 04:02:01
E: 01:01:01
|
Required products
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.
Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.
Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.
Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.



