|Description||Introducing B16-F0 cells, a valuable resource for biological research in animal cells. These cells, derived from Mus musculus (mouse), belong to the Eukaryota kingdom and the Animalia, Metazoa, Chordata, and Vertebrata phyla. They are specifically obtained from the skin tissue of mice and classified as spindle-shaped and epithelial-like cells, presenting a mixture of both morphologies. B16-F0 cells are significant in studying melanoma, a highly malignant skin cancer. With an estimated 55,100 new invasive cases diagnosed annually in the USA, and a corresponding 7,910 fatalities, melanoma remains one of the deadliest cancers worldwide. Current treatment options, such as surgical intervention, often fall short in advanced and metastatic cases, necessitating the development of improved therapeutic approaches. In biological research, B16-F0 cells serve as a valuable model for studying metastasis and solid tumour formation, making them instrumental in advancing our understanding of these critical processes. They have proven to be practical tools in investigating the biology of melanoma and have been extensively utilized in preclinical studies focused on developing new therapeutic strategies. One particular application of B16-F0 cells is their use in 3D cell culture systems. The ability to grow these cells in a three-dimensional environment mimics the in vivo conditions more closely, allowing researchers better to study the complex interactions and behaviour of melanoma cells. This approach enhances the relevance and translatability of experimental findings, providing valuable insights into melanoma progression and potential therapeutic targets. B16-F0 cells offer researchers a versatile and highly relevant model for investigating melanoma and its associated processes. Their unique characteristics, derived from mouse skin tissue, make them an invaluable resource for understanding tumour biology, developing new therapeutic approaches, and combating melanoma's devastating impact.|
|Morphology||mixture of spindle-shaped and epithelial-like cells|
Identifiers / Biosafety / Citation
|Citation||B16-F0 (Cytion catalog number 300308)|
Expression / Mutation
|Tumorigenic||Yes, in syngeneic mice|
|Medium supplements||10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate|
|Freeze medium||CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)|
|Handling of cryopreserved cultures||The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.|
|Handling of proliferating cultures||One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.|
Quality control / Genetic profile / HLA
|Sterility||Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.|
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.