FRhK-4

€375.00*

Product number: 305151

Description	FRhK-4 cells, a remarkable epithelial-like cell line, were derived from the kidney of a female rhesus monkey (Macaca mulatta). This valuable cell line was generously deposited by JC Petricciani, renowned for his contributions to the field of virology. With an impressive rating of 99 out of 100 Bioz Stars and a remarkable 212 product citations, FRhK-4 cells have proven to be a trusted resource for numerous researchers. These cells possess a distinct epithelial morphology, closely resembling the structural characteristics of kidney tissue. Originating from a typical sample obtained from a fetal rhesus monkey, FRhK-4 cells represent an ideal model for studying various biological phenomena in a controlled environment. One of the notable applications of FRhK-4 cells lies in their compatibility with 3D cell culture techniques. With the ability to form three-dimensional structures, these cells offer an excellent platform for investigating complex cellular processes, tissue development, and organogenesis. Researchers can utilize FRhK-4 cells to explore cell-cell interactions, matrix remodelling, and the effects of external factors in a more physiologically relevant setting. FRhK-4 cells have garnered particular attention in virology as a non-human primate cell line. They have been identified as susceptible to infection by different poliovirus types, including type 1, type 2, and type 3. This susceptibility allows scientists to study the pathogenesis of poliovirus and develop potential therapeutic strategies. Derived from the fetal kidney, FRhK-4 cells hold immense promise as an invaluable resource for biological research. Their epithelial-like nature and susceptibility to viral infection provide a unique platform to investigate cellular behaviour and viral pathogenesis. Whether exploring 3D cell culture or investigating viral infections, FRhK-4 cells are an indispensable tool for advancing our understanding of complex biological systems.
Organism	Rhesus macaque
Tissue	Embryonic kidney
Synonyms	FRHK-4, Frhk-4, FRhK4, Fetal Rhesus Kidney-4

Age	Fetus
Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Citation	FRhK-4 (Cytion catalog number 305151)
Biosafety level	1

Culture Medium	DMEM
Medium supplements	10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solution	Accutase
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

FRhK-4

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA