HEK293 EBNA

€375.00*

Product number: 300264

Description	Large-scale transfection of mammalian cells has emerged as an indispensable technology within the scientific community. It allows for the efficient production of milligram-to-gram quantities of r-proteins within a short timeframe, just a few days after the cloning of cDNAs into the suitable expression vector. Among the various cell lines available, the HEK293 cell line stably expressing the Epstein-Barr virus nuclear antigen-1 (HEK293-EBNA1, or 293E) stands out as the most widely utilized cell line for large-scale transfection experiments. One of the critical advantages of utilizing HEK293-EBNA1 cells is their compatibility with expression vectors carrying the Epstein-Barr virus origin of replication, oriP, such as the pTT vector. This compatibility leads to a significant threefold improvement in the yield of r-proteins compared to using a non-oriP vector. Leveraging this potential, researchers can now achieve higher yields of their desired r-proteins, bringing them closer to their research and development goals.
Organism	Human
Tissue	Embryonic kidney
Synonyms	HEK293-EBNA, 293 c18, 293c18, HEK 293 c18, HEK-293 c18, HEK293-EBNA1, HEK-293-EBNA, HEK 293-EBNA, HEK 293 EBNA, HEK293EBNA, 293 EBNA, 293-EBNA1, 293-EBNA, 293/EBNA, 293EBNA, EBNA-293, EBNA293, HEK293E, HEK/EBNA, HEK-EBNA, HEK.EBNA, 293/EBNA-1

Age	Fetus
Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Citation	HEK293 EBNA (Cytion catalog number 300264)
Biosafety level	2

Antigen expression	EBNA1
Viruses	Adenovirus 5 (Transformant), EBV (expresses EBNA1)

Culture Medium	DMEM
Medium supplements	10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solution	Accutase
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

HEK293 EBNA

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA