Hey

€375.00*

Product number: 305017

Description	HEY Cells: An Essential Tool for Cancer Research HEY Cells, derived from a human ovarian cancer xenograft, are a valuable resource for cancer researchers seeking to advance their understanding of papillary cystadenocarcinoma, a moderately differentiated form of ovarian cancer. The parental cell line, HEY, was initially obtained from a peritoneal sample of a Caucasian patient diagnosed with this specific type of cancer. A derivative known as HEY-T30 (CRL-3252) was developed through a carefully designed process to enhance the versatility and usefulness of HEY Cells. This involved subjecting the parental HEY cell line to stepwise escalating concentrations of Taxol for six months. HEY-T30 is now maintained in a medium containing 30 nmol/L Taxol, ensuring its stability and consistency. It is worth noting that Jurriaan Brouwer-Visser Institution is the designated repository for this cell line. These epithelial-like cells closely resemble human cells, making them an excellent model for studying ovarian cancer. HEY, Cells exhibit a rapid doubling time of approximately 30 hours, allowing for efficient and time-effective experimentation. Researchers can utilize these cells to investigate various aspects of cancer biology, such as tumour formation, metastasis, and drug response. HEY, Cells are particularly well-suited for applications involving 3D cell culture, a technique that more closely mimics the physiological environment of tumours. Their ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice highlights their adaptability and potential for in vivo studies. By incorporating HEY Cells into cancer research, scientists can uncover crucial insights into the development and progression of papillary cystadenocarcinoma. These cells are invaluable for exploring novel therapeutic strategies, identifying potential drug targets, and evaluating treatment efficacy. In summary, HEY Cells provide researchers with a robust and reliable resource for investigating ovarian cancer. With their origins in a patient sample and their epithelial-like morphology, these cells faithfully replicate key characteristics of papillary cystadenocarcinoma. Their applications in 3D cell culture and cancer research make them essential in advancing our understanding of this challenging disease.
Organism	Human
Tissue	Ovary
Disease	High grade ovarian serous adenocarcinoma
Synonyms	HEY

Age	Unspecified
Gender	Female
Ethnicity	European
Morphology	Epithelial
Growth properties	Adherent

Citation	Hey (Cytion catalog number 305017)

Tumorigenic	Yes

Culture Medium	DMEM
Medium supplements	10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solution	Accutase
Doubling time	20 to 30 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	1:3 to 1:5
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.
STR profile	Amelogenin: x,x CSF1PO: 10,11 D13S317: 11 D16S539: 8,12 D5S818: 11,12 D7S820: 12 TH01: 8,9.3 TPOX: 11 vWA: 16,17 D3S1358: 16 D21S11: 30 D18S51: 15 Penta E: 7,13 Penta D: 9,13 D8S1179: 13 FGA: 20,21 D6S1043: 11,12 D2S1338: 24,25 D12S391: 17,22 D19S433: 13,14

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Hey

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA