HuH-6

€375.00*

Product number: 305092

Description	HuH-6 cells, short for Human Hepatoma 6 cells, are a permanent cell line established from hepatoma tissue obtained from a 57-year-old Japanese male in 1982. These cells have become a valuable tool in biological research, providing a convenient substitute for primary hepatocytes. The primary application of HuH-6 cells lies in studying hepatitis C virus (HCV) infection and hepatoma. Researchers often use these cells as a cell culture model to investigate the mechanisms of HCV infection and explore potential treatments for hepatoma. One crucial area of study involving HuH-6 cells is understanding drug metabolism and pharmacokinetics. These cells are commonly used to assess the potential for drug-drug interactions (DDIs) by analyzing the gene expression of various CYP450 enzymes. Regulatory agencies require an evaluation of DDI potential in hepatocytes for specific enzymes like CYP3A4, CYP1A2, and CYP2B6. HuH-6 cells, known as Cryopreserved Human Hepatocytes, Induction qualified (HUCPI), have been characterized to exhibit enzyme induction levels recommended by the FDA for evaluating DDIs. Another significant application of HuH-6 cells is the study of transporter-mediated efflux inhibition. Plating the hepatocytes onto collagen and overlaying them with a basement membrane extract allows the formation of polarized cell surface structures and the re-expression of bile efflux transporters. This enables researchers to investigate the effects of drug inhibition on bile efflux transport, which can range from drug-drug interactions to liver toxicity caused by the accumulation of intracellular bile acids. Hepatocytes pre-characterized for transporter function are well-suited for these studies. Additionally, HuH-6 cells play a crucial role in the pharmaceutical discovery of drugs with a slower metabolism. By measuring the basic metabolic properties of drugs that are metabolized at a slower rate, researchers can develop medications that remain effective for more extended periods. Plated hepatocytes, like HuH-6 cells, maintain their metabolic activity for an extended duration, making them ideal for assays lasting four hours or longer. Hepatocytes prequalified for low clearance are especially valuable for these studies, although Induction or Transporter qualified hepatocytes (HUCPI) can also be used. HuH-6 cells are a valuable cell line derived from hepatoma tissue. They are commonly used in biological research to study hepatitis C virus infection, hepatoma, drug metabolism and pharmacokinetics, drug-drug interactions, transporter-mediated efflux inhibition, and the discovery of drugs with a slower metabolism. These cells provide researchers with a convenient and reliable model to investigate various aspects of liver-related biology and drug development.
Organism	Human
Tissue	Liver
Disease	Hepatoblastoma
Synonyms	HUH-6, HuH 6, HuH6, HUH6, Huh6

Age	1 year
Gender	Male
Ethnicity	Asian
Morphology	Epithelial
Growth properties	Adherent

Citation	HuH-6 (Cytion catalog number 305092)
Biosafety level	1

Culture Medium	DMEM
Medium supplements	10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solution	Accutase
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.
STR profile	Amelogenin: x,Y CSF1PO: 10,12 D13S317: 8,12 D16S539: 10,11 D5S818: 8,11 D7S820: 11,12 TH01: 7,8 TPOX: 8 vWA: 14,17 D3S1358: 14,17 D21S11: 29,30 D18S51: 13,21 Penta E: 11 Penta D: 9,13 D8S1179: 10,11 FGA: 19,24 D6S1043: 13,18 D2S1338: 18 D12S391: 18,20 D19S433: 12,12.2

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

HuH-6

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA