Jurkat

€375.00*

Prices excl. VAT plus shipping costs

Product variants

cryopreserved culture

Product number: 302147

Safety data sheet

Product sheet

Description	Jurkat cells, a human T-cell acute lymphoblastic leukemia (T-ALL) cell line, were created in the 1980s from the peripheral blood of a 14-year-old male T-ALL patient. The cell line has been used extensively to study T-cell biology and develop T-cell therapies. The Jurkat cell line is hypotetraploid with a flat modal karyotype of 46 chromosomes and 7.8% polyploidy. Jurkat cells express T-cell receptor (TCR) and CD3 proteins. They also express CD4 and CD8 co-receptors, which aids in identifying them as helper or cytotoxic T cells. The Jurkat cells have a point mutation in the Lck gene, which encodes a protein necessary for T-cell activation, causing T cells to be constitutively activated. Jurkat cells have been used to study T-cell activation and signaling, cell cycle regulation, proliferation, and differentiation. These cells have also been used to test the efficacy of drugs like kinase inhibitors and study T-molecular ALL's mechanisms. In summary, Jurkat cells are a widely used human T-cell leukemia cell line that has been used to study T-cell biology, develop T-cell-based therapies, and model cancer.
Organism	Human
Tissue	Blood
Disease	T-cell acute lymphoblastic leukemia
Metastatic site	Peripheral blood
Synonyms	JURKAT, JM, JM-Jurkat, Jurkat-FHCRC, Jurkat FHCRC, FHCRC-11, FHCRC subclone 11, FCCH1024

Age	14 years
Gender	Male
Ethnicity	European
Morphology	Lymphoblast
Growth properties	Suspension

Citation	Jurkat (Cytion catalog number 302147)
Biosafety level	1

Culture Medium	IMEM
Medium supplements	10% FBS, w: 0.05 mM 2-mercaptoethanol, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3
Passaging solution	The utilization of a passaging solution is not necessary when passaging cells that are cultured in suspension. The appropriate procedure is to dilute the cells in accordance with the indicated guidelines.
Doubling time	26 hours
Subculturing	Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.
Split ratio	1:2 to 1:5
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Jurkat

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA