NCI-H1299

€375.00*

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Product variants

cryopreserved culture

Product number: 300485

Safety data sheet

Product sheet

Description	NCI-H1299, also known as H1299, is a cell line established from a lymph node metastasis of the lung from a 43-year-old white male patient with carcinoma. H1299 and H292 are non-small cell lung cancer (NSCLC) cell lines. Regarding their genetic profile, H1299 cells have a homozygous partial deletion of the p53 protein and lack expression of p53 protein. While KRAS mutations are commonly found in various types of cancer, including NSCLC, H1299 expresses KRAS WT. A549 is another NSCLC cell line that homozygously expresses endogenous KRAS G12S. Understanding the biology of KRAS and its downstream signalling pathways is crucial for developing effective cancer therapies. Therefore, this epithelial-like cell line is commonly used in cancer and immuno-oncology research. The morphology of H1299 cells is characterized by adherent flattened cells with a thickness of fewer than 5 microns. H1299 cells have an approximate doubling time of 22 - 30 hours. H1299 cells express keratin and vimentin but are negative for neurofilament triplet protein. They are also reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein but not the gastrin-releasing peptide (GRP). Compared to A549 cells with more epithelial characteristics, H1299 cells have more mesenchymal characteristics and less effective epithelial marker expression.
Organism	Human
Tissue	Lung
Disease	Carcinoma
Synonyms	H1299, H-1299, NCIH1299

Age	59 years
Ethnicity	Caucasian
Growth properties	Adherent

Citation	H1299 (Cytion catalog number 300485)
Biosafety level	1

Culture Medium	RPMI 1640
Medium supplements	10% FBS, 2.0 mM L-glutamin, 4500 mg/L glucose, 1500 mg/L NaHCO3, 1 mM sodium pyruvate, 10 mM HEPES
Passaging solution	Accutase
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

NCI-H1299

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA