NRK

€375.00*

Prices excl. VAT plus shipping costs

Product number: 305195

Safety data sheet

Product sheet

Description	The NRK cell line, derived from a Rattus norvegicus (rat) kidney, is an invaluable tool in biological research. These cells possess an epithelial morphology, meaning they form sheets covering the organs' surfaces and protecting against foreign substances. Epithelial cells, like NRK cells, exhibit specific characteristics. They have a generous amount of cytoplasm and contain numerous granules. These cells serve various bodily functions, with some acting as absorptive or protective agents while others act primarily as secretory cells. In the case of the kidneys, the epithelial cells play a crucial role in the storage and subsequent secretion of excretory materials. This makes the NRK cell line particularly suitable for studying renal physiology. By utilizing these cells, researchers can investigate the intricate processes involved in kidney function and gain insights into various aspects of renal physiology. Moreover, the NRK cell line is not limited to studying renal physiology alone. These versatile cells can also be employed in cancer research. Their epithelial morphology and origin from a normal rat kidney make them an excellent model for investigating the behaviour and characteristics of cancer cells in a controlled environment. One application that leverages the unique properties of NRK cells is 3D cell culture. This technique involves growing cells in a three-dimensional matrix miming the natural cellular environment more closely than traditional two-dimensional culture. NRK cells can be cultured in this manner, allowing researchers to create complex tissue models that closely resemble the native structure of the kidney. This facilitates the study of cellular behaviour, interactions, and responses in a more physiologically relevant context. The NRK cell line is a valuable resource in biological research, specifically in cancer and renal physiology. These epithelial cells, derived from the kidney of an average rat, offer researchers the opportunity to delve into the intricacies of renal function and study cancer cells in a controlled laboratory setting. With their applicability in 3D cell culture, NRK cells enable the creation of realistic tissue models for comprehensive investigations into cellular behaviour and responses.
Organism	Rat
Tissue	Kidney
Synonyms	Normal Rat Kidney

Age	Adult
Morphology	Epithelial
Growth properties	Adherent

Citation	NRK (Cytion catalog number 305195)
Biosafety level	1

Culture Medium	DMEM
Medium supplements	10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging solution	Accutase
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contaminations are ruled out through PCR-based and luminescence-based mycoplasma assays. The absence of bacterial, fungal or yeast contamination is controlled through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

NRK

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA