- Phenol-red free
- No
- Serum content
- Serum-free
- Recommended
- 6,000 cells/cm² (initial seeding up to approx. 90 % confluence)
Documents
Defined Fibroblast Maintenance Medium
Product · Product number 850205
Serum-free, defined medium for the maintenance culture of human fibroblasts
01Description
Defined Fibroblast Maintenance Medium is a serum-free, fully defined medium that keeps human fibroblasts in a stable state rather than driving rapid expansion. It suits secretome, extracellular matrix and compound testing work where serum would interfere. The complete medium contains L-glutamine, human recombinant bFGF and human recombinant insulin. For the expansion of fibroblasts, choose Fibroblast Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the serum-free supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately.
02Product details
- Phenol-red free
- No
- Serum content
- Serum-free
- Recommended
- 6,000 cells/cm² (initial seeding up to approx. 90 % confluence)
- Storage and stability
- Store the basal medium at 2–8 °C, protected from light, and the supplements at −20 °C. Avoid repeated freeze-thaw cycles of the supplements. Once supplemented, the medium can be kept at 2–8 °C, protected from light, for up to one month. Do not freeze the medium.
- Specification
- pH 7.6, osmolality 285 ± 10 mOsm/kg
- Supplement components
- L-glutamine: 4 mM Human recombinant bFGF: 1 ng/ml Human recombinant insulin: 5 µg/ml Antibiotics (optional): gentamicin 50 µg/ml, amphotericin B 50 ng/ml
- Composition table
Supplement volumes for 500 ml of basal medium. The same components are also available premixed in one bottle (Supplement-Mix, Cat. No. 850164, 11 ml).
Component
Volume
Cat. No.
L-glutamine
10 ml
850121
Human recombinant bFGF
0.5 ml
850138
Human recombinant insulin
0.5 ml
850140
Antibiotics (optional)
3.5 ml
850119
Total (without antibiotics)
11 ml