KLE

The KLE cell line is an adherent cell line derived from the endometrium of a White, female patient with adenocarcinoma. This cell line was established from a 64-day-old patient and has since become a vital tool in endometrial cancer research. KLE cells were deposited by GR Richardson and are known for their tumorigenic properties, as they form tumors within 21 days with 100% frequency when inoculated subcutaneously in nude mice. These tumors do not form glands but exhibit microvilli, junctional complexes, and nucleolar channel systems similar to those found in normal endometrium under progestational stimulation.

KLE cells express blood type O and are Rh-positive, which can be relevant for specific studies involving antigen expression. The cell line is commonly used to study the pathophysiology of endometrial carcinoma, with particular interest in its estrogen receptor-negative and progesterone receptor-positive status. This receptor profile makes KLE cells highly suitable for research into progesterone's role in endometrial cancer progression. Electron microscopy studies of KLE cell-derived tumors have provided detailed insights into the cellular ultrastructure, making this cell line an essential resource for understanding the morphological aspects of endometrial adenocarcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.