- Serum content
- 5 % FCS (25 ml FCS per 500 ml basal medium)
- Recommended
- 4,000 cells/cm² (initial seeding up to approx. 90 % confluence)
Documents
Microvascular Endothelial Cell Growth Medium
Product · Product number 850212
Medium for microvascular endothelial cells, with ECGS/H
01Description
Microvascular Endothelial Cell Growth Medium is used for the culture of human microvascular endothelial cells, for example from dermis, lung or heart. The complete medium contains foetal calf serum, L-glutamine, ECGS/H (endothelial cell growth supplement with heparin), EGF and hydrocortisone. For defined recombinant growth factors including VEGF, choose Advanced Microvascular Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).
02Product details
- Serum content
- 5 % FCS (25 ml FCS per 500 ml basal medium)
- Recommended
- 4,000 cells/cm² (initial seeding up to approx. 90 % confluence)
- Storage and stability
- Store the basal medium at 2–8 °C, protected from light, and the supplements at −20 °C. Avoid repeated freeze-thaw cycles of the supplements. Once supplemented, the medium can be kept at 2–8 °C, protected from light, for up to one month. Do not freeze the medium.
- Specification
- pH 7.6, osmolality 285 ± 10 mOsm/kg
- Supplement components
- L-glutamine: 4 mM FCS: 5 % (25 ml per 500 ml basal medium) ECGS/H (endothelial cell growth supplement with heparin): 12 µg/ml Human recombinant EGF: 1 ng/ml Hydrocortisone: 1 µg/ml Antibiotics (optional): gentamicin 50 µg/ml, amphotericin B 50 ng/ml
- Composition table
Supplement volumes for 500 ml of basal medium. The same components are also available premixed in one bottle (Supplement-Mix, Cat. No. 850159, 38 ml).
Component
Volume
Cat. No.
L-glutamine
10 ml
850121
FCS
25 ml
850108
ECGS/H (endothelial cell growth supplement with heparin)
2 ml
850122
Human recombinant EGF
0.5 ml
850113
Hydrocortisone
0.5 ml
850130
Antibiotics (optional)
3.5 ml
850119
Total (without antibiotics)
38 ml