- Phenol-red free
- No
- Serum content
- 5 % FCS (25 ml FCS per 500 ml basal medium)
- Recommended
- 4,000 cells/cm² (initial seeding up to approx. 90 % confluence)
Documents
Skeletal Muscle Cell Growth Medium
Product · Product number 850216
Medium for the expansion of human skeletal myoblasts
01Description
Skeletal Muscle Cell Growth Medium keeps human skeletal myoblasts proliferating and avoids premature fusion, so the cells can be expanded before differentiation. The complete medium contains foetal calf serum, L-glutamine, fetuin, EGF, bFGF, human recombinant insulin and dexamethasone. For fusion into myotubes, switch to Skeletal Muscle Cell Differentiation Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).
02Product details
- Phenol-red free
- No
- Serum content
- 5 % FCS (25 ml FCS per 500 ml basal medium)
- Recommended
- 4,000 cells/cm² (initial seeding up to approx. 90 % confluence)
- Storage and stability
- Store the basal medium at 2–8 °C, protected from light, and the supplements at −20 °C. Avoid repeated freeze-thaw cycles of the supplements. Once supplemented, the medium can be kept at 2–8 °C, protected from light, for up to one month. Do not freeze the medium.
- Specification
- pH 7.6, osmolality 285 ± 10 mOsm/kg
- Supplement components
- L-glutamine: 4 mM FCS: 5 % (25 ml per 500 ml basal medium) Human recombinant EGF: 10 ng/ml Human recombinant bFGF: 1 ng/ml Human recombinant insulin: 10 µg/ml Fetuin: 50 µg/ml Dexamethasone: 0.4 µg/ml Antibiotics (optional): gentamicin 50 µg/ml, amphotericin B 50 ng/ml
- Composition table
Supplement volumes for 500 ml of basal medium. The same components are also available premixed in one bottle (Supplement-Mix, Cat. No. 850166, 38 ml).
Component
Volume
Cat. No.
L-glutamine
10 ml
850121
FCS
25 ml
850108
Human recombinant EGF
1 ml
850115
Human recombinant bFGF
0.5 ml
850138
Human recombinant insulin
0.5 ml
850141
Fetuin
0.5 ml
850132
Dexamethasone
0.5 ml
850133
Antibiotics (optional)
3.5 ml
850119
Total (without antibiotics)
38 ml