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Cytion Chondrocyte growth medium, kit with 500 ml basal medium bottle plus supplement tubes, REF 850048
Serum content
10 % FCS (50 ml FCS per 500 ml basal medium)
Recommended
4,000 cells/cm² (initial seeding up to approx. 90 % confluence)

Documents

Chondrocyte Growth Medium

Product · Product number 850204

Medium for the monolayer expansion of human and bovine chondrocytes

€310.00excl. tax and shipping
In stock
1

01Description

Chondrocyte Growth Medium is used for the monolayer expansion of human and bovine chondrocytes, for example before transfer into pellet or scaffold culture. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

02Product details

Serum content
10 % FCS (50 ml FCS per 500 ml basal medium)
Recommended
4,000 cells/cm² (initial seeding up to approx. 90 % confluence)
Storage and stability
Store the basal medium at 2–8 °C, protected from light, and the supplements at −20 °C. Avoid repeated freeze-thaw cycles of the supplements. Once supplemented, the medium can be kept at 2–8 °C, protected from light, for up to one month. Do not freeze the medium.
Specification
pH 7.6, osmolality 285 ± 10 mOsm/kg
Supplement components
HEPES: 20 mM L-glutamine: 4 mM FCS: 10 % (50 ml per 500 ml basal medium) Antibiotics (optional): gentamicin 50 µg/ml, amphotericin B 50 ng/ml
Composition table

Supplement volumes for 500 ml of basal medium. The same components are also available premixed in one bottle (Supplement-Mix, Cat. No. 850161, 70 ml).

Component

Volume

Cat. No.

L-glutamine

10 ml

850121

FCS

50 ml

850110

HEPES

10 ml

850120

Antibiotics (optional)

3.5 ml

850119

Total (without antibiotics)

70 ml