BS-C-1

The BS-C-1 cell line, also known as Cercopithecus aethiops kidney cells, originates from the kidney of the African green monkey. This cell line, established in the 1960s, is used extensively in virology research due to its susceptibility to adenoviruses, simian viruses, and other pathogenic agents. BS-C-1 cells exhibit epithelial morphology and are adherent in culture, making them suitable for a variety of experimental setups, including virus-host interaction studies and cytotoxicity assays.

One of the distinguishing features of BS-C-1 cells is their utility in the propagation and maintenance of polioviruses, which facilitates vaccine development and virus lifecycle studies. The cells are also known for their role in the discovery and study of adenoviruses, contributing significantly to our understanding of viral genetics and replication processes. Despite their origins and primary uses, BS-C-1 cells have also been employed in pharmacological research and toxicology, testing the effects of various substances on cellular processes and viability.

Due to their robust growth characteristics and ability to be transfected relatively easily, BS-C-1 cells are valuable in molecular biology for gene expression studies. Their compatibility with a wide range of DNA transfection methods supports their use in gene therapy research and recombinant protein production. Overall, BS-C-1 cells continue to be a critical resource in biomedical research, providing insights into cellular behavior and the molecular basis of disease.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.