CASP-2-1

The CASP-2-1 cell line is a genetically defined mouse prostate epithelial cell line developed from an androgen-dependent primary tumor using a tissue recombination approach. CASP-2-1 is part of the CASP series of prostate cancer models, which were generated through recombination of dissociated embryonic urogenital sinus mesenchyme with prostate tumor epithelial cells, allowing for in vivo ductal formation and validation of epithelial identity. CASP-2-1 cells express epithelial markers such as E-cadherin and demonstrate stable proliferation over at least 30 passages, with consistent tumorigenic capacity demonstrated by anchorage-independent growth in vitro and tumor formation after orthotopic injection in mice.

A defining feature of CASP-2-1 is its retention of androgen responsiveness. Unlike several other CASP lines, CASP-2-1 shows growth dependence on androgens and exhibits activation of androgen-responsive promoter elements in the presence of dihydrotestosterone (DHT). It expresses wild-type androgen receptor (AR) at both the mRNA and protein levels without detectable AR mutations. Additionally, CASP-2-1 is heterozygous for the Pten gene and shows moderate levels of phosphorylated Akt (p-Akt), indicating partial activation of the PI3K/Akt signaling pathway. These features position CASP-2-1 as a valuable model for dissecting interactions between AR signaling and Pten-dependent oncogenic pathways in prostate cancer, particularly in early-stage or androgen-sensitive disease contexts.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.