LC-540

The LC-540 cell line is an adherent cell model derived from an adult male Fischer rat. Known for its robust growth properties, this cell line has a modal chromosome number of 42, with a karyotypic range of 40 to 43. Approximately 21% of the cells exhibit aneuploidy, although no other structural abnormalities have been reported, indicating a relatively stable genomic profile.

LC-540 cells are tumorigenic, with the ability to form tumors when introduced into rats. This feature makes them particularly valuable for studying oncogenesis and tumor biology in a controlled in vitro environment. Additionally, these cells are susceptible to several viruses, including Herpes simplex virus, Vaccinia virus, Vesicular stomatitis virus, and Human poliovirus 1. This susceptibility renders LC-540 a useful model for virological research, particularly in exploring virus-host interactions, viral pathogenesis, and the development of antiviral strategies.

Due to their specific characteristics, LC-540 cells are instrumental in a range of research applications, including cancer research and virology, where they provide insights into the mechanisms of tumor formation and viral infections.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.