HEC-1-B

The HEC-1-B cell line is a human endometrial adenocarcinoma cell line. This line has been utilized extensively in biomedical research related to the study of endometrial cancer, hormone responses, and cancer pharmacology. The cells are known to express estrogen and progesterone receptors, making them a valuable model for studying hormone-related dynamics in endometrial cancer progression and treatment. These cells have been used to investigate the molecular mechanisms of cancer cell proliferation, differentiation, and response to hormonal and chemotherapeutic treatments.

In terms of morphology, HEC-1-B cells typically exhibit an epithelial-like shape and grow in a monolayer. They are characterized by their high capacity for in vitro proliferation. Genetic studies have revealed several chromosomal alterations which are thought to contribute to the cancerous phenotype of these cells. Research using the HEC-1-B cell line has contributed to a deeper understanding of endometrial carcinogenesis and offers a robust system for testing potential therapeutic agents. This cell line is also commonly employed in studies focusing on cancer cell invasion and metastasis, providing insights into the cellular behaviors that underpin these processes.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.