JEG-3

The JEG-3 cell line is derived from a human choriocarcinoma, a type of cancer that originates from trophoblastic cells in the placenta. These cells exhibit properties characteristic of trophoblasts, including the ability to produce hormones such as human chorionic gonadotropin (hCG), which is crucial for pregnancy maintenance. JEG-3 cells are epithelial in nature and are often utilized in research focused on placental function, cancer biology, and endocrine signaling.

JEG-3 cells are known for their aggressive growth characteristics and capacity to invade surrounding tissues, making them a valuable model for studying the mechanisms of trophoblastic tumor invasion and metastasis. Additionally, they have been used extensively in research investigating the molecular pathways involved in placental development, as well as the role of trophoblasts in immune tolerance during pregnancy. The cells are typically cultured in RPMI-1640 medium supplemented with fetal bovine serum and other growth factors to support their proliferation and maintenance.

This cell line provides a robust platform for investigating placental cancer biology, hormone production, and the interaction between trophoblasts and the maternal immune system.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.