JJN-3

JJN-3 is a human multiple myeloma cell line established from the bone marrow of a 57-year-old Caucasian female patient. The line grows in suspension as single round-to-oval cells, occasionally showing multinucleated morphology. JJN-3 harbors a NRAS p.Gln61Lys activating mutation, reflecting the RAS pathway dysregulation common in myeloma. It is a plasma cell-derived myeloma line that retains the immunophenotypic characteristics of malignant plasma cells, including CD38 and CD138 expression, and has been used in numerous studies of myeloma biology and drug sensitivity.

JJN-3 is applicable in studies of multiple myeloma biology, NRAS Q61K oncogenic signalling, anti-myeloma drug evaluation (bortezomib, lenalidomide, daratumumab, elotuzumab), plasma cell differentiation, and bone marrow microenvironment interactions. It is one of the established myeloma reference lines and is suitable for studies of CD38-targeted immunotherapy, IMiD effects, and proteasome inhibitor responses in the context of RAS-mutant myeloma.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.