CEM/C1

The CEM/C1 cell line is a derivative of the CCRF-CEM human T-cell leukemia cell line, specifically selected for its resistance to certain chemotherapeutic agents, notably the topoisomerase II inhibitor, doxorubicin. This selection confers the cell line with significant applications in the study of multidrug resistance, a prevalent challenge in the treatment of various cancers. The CEM/C1 line exhibits overexpression of the MDR1 gene, which encodes the P-glycoprotein, a key efflux transporter involved in the resistance of cells to chemotherapeutic drugs.

Genetically, CEM/C1 cells are characterized by their human T-lymphoblastoid lineage, making them highly relevant for research into T-cell biology and leukemia. The cells maintain a robust proliferative capacity and can be used in in vitro experiments aimed at understanding the cellular mechanisms of drug resistance, apoptosis, and the efficacy of new chemotherapeutic agents. These cells also provide a valuable tool for pharmacological studies, particularly in evaluating the pharmacodynamics and pharmacokinetics of anticancer drugs within a controlled experimental setting.

Due to their drug-resistant properties, CEM/C1 cells are particularly useful in the development of treatment strategies that circumvent or directly target mechanisms of drug resistance. Studies utilizing this cell line can contribute to the broader understanding of cancer cell survival tactics and potentially lead to the development of more effective cancer therapies, especially for refractory or relapsed T-cell leukemia.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.