KYSE-150

The KYSE-150 cell line is a human esophageal squamous cell carcinoma (ESCC) model derived from a primary tumor resected from an adult patient. This cell line is part of the KYSE series, which was developed to provide a reliable in vitro model for studying the pathobiology of esophageal cancer, particularly in understanding tumorigenesis and therapeutic response. The KYSE-150 cells exhibit a rapid doubling time of 13.7 hours, indicating a high proliferative capacity, which is characteristic of aggressive cancer phenotypes. These cells grow in monolayer culture, adhering to the substrate and forming a uniform sheet, which is typical for epithelial-derived cancer cells.

Genetic analysis of KYSE-150 reveals significant alterations in key tumor suppressor genes, particularly the p16 (INK4a) gene. This cell line shows aberrations in the p16 gene, specifically in the form of CpG island methylation, which silences the gene and contributes to the loss of cell cycle regulation. This epigenetic modification is a common mechanism in many cancers and highlights the relevance of KYSE-150 for studying gene silencing and its role in cancer progression. Furthermore, the cell line retains the wild-type configuration of the p15 gene, suggesting a selective inactivation mechanism for p16 over p15 in this model, which may be of interest in comparative genomic studies.

KYSE-150 is not only valuable for studying the molecular and cellular mechanisms of ESCC but also for exploring the effects of genetic and epigenetic alterations in cancer. It provides a robust model for investigating therapeutic interventions that target the specific pathways dysregulated in esophageal squamous cell carcinoma. Given its high proliferation rate and specific genetic profile, KYSE-150 is a suitable candidate for in vitro pharmacological testing and other applications related to cancer research, but not for therapeutic or in vivo purposes.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.