BFTC-905

The BFTC-905 cell line is a human transitional cell carcinoma (TCC) model derived from a high-grade, papillary bladder tumor in a female patient. It was established to represent aggressive bladder cancer and has been used in cytogenetic and molecular profiling studies to understand bladder tumor biology and therapeutic vulnerabilities. BFTC-905 exhibits a highly complex and rearranged karyotype, which includes multiple chromosomal abnormalities typical of advanced bladder cancers. These include non-random alterations such as deletions of 8p, duplications of 8q, and gains in chromosomes 7 and 20, features often linked to disease progression and poor prognosis in urothelial carcinoma.

Extensive characterization using multi-color fluorescence in situ hybridization (M-FISH) has revealed numerous structural rearrangements in BFTC-905, including interchromosomal translocations and deletions affecting loci with potential relevance to tumor suppressor loss. Specifically, BFTC-905 displays a deletion of chromosome 8p21, a region frequently lost in aggressive TCC and associated with tumor suppressor genes. This cytogenetic complexity provides a valuable opportunity for dissecting gene function in the context of genomic instability, a hallmark of late-stage bladder cancers.

BFTC-905 has also been included in large-scale pharmacogenomic studies such as the Cancer Cell Line Encyclopedia (CCLE) and Genomics of Drug Sensitivity in Cancer (GDSC). These resources have confirmed the molecular fidelity of BFTC-905 to primary bladder tumors and have enabled its use in predictive modeling of anticancer drug responses. Its multi-omics profile-including gene expression, mutation status, copy number variation, and DNA methylation-makes it a powerful model for investigating bladder cancer-specific therapeutic targets and resistance mechanisms.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.