EL4

The EL4 cell line is derived from a lymphoma of a mouse and is extensively used in immunology and cancer research. These cells originate from a thymoma, a type of tumor arising from the thymic epithelial cells, and they serve as a model for studying T-cell lymphomas and the immune response. EL4 cells are valuable for investigating the mechanisms of T-cell development, activation, and signaling, as well as the interaction between tumor cells and the immune system. Due to their lymphoid origin, EL4 cells are also employed in research focused on the production and function of cytokines, which are critical for immune regulation.

EL4 cells display a lymphoblastic morphology and express markers characteristic of T-cells, such as CD3 and T-cell receptor complexes. They are highly responsive to various stimuli that activate T-cells, making them suitable for studies on T-cell receptor signaling pathways and the effects of immunomodulatory agents. Furthermore, EL4 cells are used in tumor immunology to explore the interactions between cancer cells and the immune system, aiding in the development of immunotherapies for T-cell lymphomas and other cancers. The ability of EL4 cells to produce large quantities of specific cytokines, such as interleukin-2 (IL-2), makes them a useful tool in both basic research and the development of therapeutic strategies targeting immune responses.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.