KG-1

KG-1 is a human acute myelogenous leukemia (AML) cell line derived from the bone marrow of an adult patient with erythroleukemia. This cell line is a valuable model for studying hematopoietic differentiation and leukemia, particularly due to its unique characteristics, including the expression of several hematopoietic markers. KG-1 cells are classified as immature myeloid cells that resemble early progenitor cells, which makes them a useful tool for investigating the early stages of myeloid lineage commitment and the molecular mechanisms driving leukemogenesis.

KG-1 cells exhibit a high degree of plasticity, which allows them to differentiate into various hematopoietic lineages under the right experimental conditions. This characteristic is particularly important for research in understanding the regulation of hematopoiesis and the development of therapeutic strategies aimed at targeting leukemic stem cells. Additionally, KG-1 cells are known to express markers such as CD34, HLA-DR, and CD13, which are critical in both normal and malignant hematopoiesis, making them an excellent model for flow cytometry and other immunophenotyping studies.

KG-1 has also been employed in drug discovery and toxicity testing, where its responsiveness to differentiation agents and chemotherapeutic drugs can be evaluated. As with all in vitro models, it is important to recognize that KG-1 cells are for research use only and not suitable for therapeutic or in vivo applications.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.