CASP-1-1

The CASP-1-1 cell line is a genetically engineered mouse prostate epithelial cell model, part of the CASP series established from primary, androgen-dependent prostate tumors in mice. These cells were derived using tissue recombination methods involving the urogenital sinus mesenchyme (UGM), enabling the formation of prostatic ducts in vivo, thus confirming their epithelial identity. CASP-1-1 cells express epithelial markers such as E-cadherin and demonstrate tumorigenic potential through anchorage-independent growth in vitro and tumor formation upon orthotopic injection into mouse prostates. They are immortalized and stable in long-term culture, having been maintained for at least 30 passages.

Genetically, CASP-1-1 cells are characterized by a heterozygous Pten status (+/−) and show medium levels of activated Akt (p-Akt), reflecting functional PI3K/Akt signaling alterations common in prostate cancer models. The androgen receptor (AR) is both transcriptionally and translationally expressed in these cells, and sequencing has revealed no mutations in the AR coding region. However, despite expressing wild-type AR, CASP-1-1 is androgen-independent, meaning its proliferation is not reliant on exogenous androgens, and it does not exhibit androgen-responsive promoter activity under standard assay conditions. This makes CASP-1-1 particularly useful for studying the transition from androgen-dependent to androgen-independent prostate cancer and the role of Pten/PI3K/Akt and AR signaling in this progression.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.