CC531

CC531 is a well-characterized rat adenocarcinoma cell line derived from the colon. It was originally established from a chemically induced colon tumor in a Wistar rat using 1,2-dimethylhydrazine (DMH), a potent carcinogen. The CC531 cell line is commonly utilized as a model system to study colorectal cancer mechanisms and the tumor microenvironment in vivo, particularly within the context of metastasis and immune responses. These cells are immunogenic and are often used in syngeneic rat models to investigate the efficacy of cancer immunotherapies and the interaction between cancer cells and the immune system.

In research settings, CC531 cells are employed to examine the biological processes of colorectal cancer progression, including cell proliferation, apoptosis, and metastatic behavior. The cell line has been instrumental in studying the response of colorectal cancer to various chemotherapeutic agents and radiation therapy, providing insights into the mechanisms of resistance and sensitivity to cancer treatments. Moreover, the CC531 model serves as a valuable tool for the development and optimization of novel therapeutic strategies targeting colorectal cancer, making it crucial for translational cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.