D341Med

The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue extracted from a 3-year-old boy diagnosed with medulloblastoma. Medulloblastoma is a highly malignant pediatric brain tumor predominantly occurring in the cerebellum. This cell line is crucial for research due to its origin from a common type of childhood brain cancer, providing insights into the tumor biology and genetics specific to pediatric cases. D341 Med has been extensively utilized in studies aimed at understanding the molecular and cellular mechanisms of medulloblastoma, including investigations into the genetic mutations and signaling pathways that contribute to tumorigenesis and treatment resistance.

In addition to its role in basic research, the D341 Med cell line has been instrumental in preclinical studies assessing new therapeutic approaches for medulloblastoma. Its genetic profile, which reflects common alterations seen in human tumors, makes it an excellent model for evaluating the efficacy of potential drugs and novel therapeutic strategies. The use of D341 Med in these studies helps to bridge the gap between laboratory research and clinical application, supporting the development of targeted therapies that could offer improved outcomes for children affected by this devastating disease.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.