HEK293-GPRC5D

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HEK293-GPRC5D cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human G protein-coupled receptor family C group 5 member D (GPRC5D), an orphan receptor belonging to the class C G protein-coupled receptor family. GPRC5D exhibits highly restricted expression in normal tissues, with predominant expression reported in hair follicles, keratinized tissues, and plasma cells. Importantly, the receptor is highly expressed in multiple myeloma and certain other plasma cell malignancies, where its limited normal tissue distribution and robust tumor-associated expression have made it a prominent target for immunotherapeutic development, particularly in patients relapsing after BCMA-directed therapies.

HEK293-GPRC5D cells are widely used in hematologic oncology research and therapeutic development for characterization of GPRC5D-targeted monoclonal antibodies, bispecific T-cell engagers, antibody-drug conjugates, and CAR-T or CAR-NK cell therapies. The stable recombinant expression system enables quantitative evaluation of antigen binding affinity, receptor occupancy, surface expression density, epitope specificity, and target-dependent cytotoxicity. These cells are particularly valuable for assessing the activity and selectivity of engineered immune cell therapies and T-cell redirecting biologics targeting GPRC5D-positive malignancies. Additional applications include flow cytometry assay development, reporter assays, high-throughput screening, and validation of receptor-specific imaging agents or diagnostic reagents.

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.