JHH-7

JHH-7 is a human hepatocellular carcinoma (HCC) cell line established from a primary HCC of a 53-year-old Japanese male patient, also designated FLC-7 (Functional Liver Cell-7). The line harbors a TP53 p.Arg267Pro mutation (unspecified zygosity), which affects the DNA-binding domain of p53 and impairs its tumor suppressor function. JHH-7 is one of several JHH-series HCC lines derived from Japanese patients at Jikei University, providing a well-characterized panel of HCC models with different TP53 mutational backgrounds and metabolic profiles.

JHH-7 is applicable in hepatocellular carcinoma research, including studies of HCC metabolism, hepatocyte differentiation markers, TP53 mutant tumor phenotype, drug sensitivity and resistance to sorafenib, lenvatinib, and other systemic HCC therapies, and comparative analyses within the JHH-series HCC panel. Its retained expression of liver-specific functions (albumin, AFP) makes it a relevant model for studying differentiated HCC biology. It is also applicable in xenograft studies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.