K7M2 wt

The K7M2 wt cell line is derived from a murine osteosarcoma and is frequently utilized in cancer research, particularly for studies investigating the pathogenesis and therapeutic response of osteosarcoma. This cell line is characterized by its high metastatic potential, making it an invaluable model for studying the mechanisms underlying cancer metastasis and for testing anti-metastatic agents. K7M2 wt cells display a typical epithelial morphology and exhibit robust growth in vitro, which facilitates various experimental applications including gene expression studies, drug screening, and genetic manipulation.

Researchers leverage the K7M2 wt cell line to explore the molecular and cellular processes involved in osteosarcoma progression. Studies often focus on signaling pathways, such as the Wnt/β-catenin and PI3K/AKT pathways, which are crucial in tumor growth and metastasis. The genetic profile of K7M2 wt cells includes alterations common in osteosarcoma, providing insights into the genetic drivers of this malignancy. Furthermore, this cell line is instrumental in preclinical testing of new therapeutic approaches, including targeted therapies and immunotherapies, offering a platform for translating research findings into potential clinical applications.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.