Primary Cell Culture Media

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Cytion Endothelial cell growth medium, advanced, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850006

Advanced Endothelial Cell Growth Medium

Advanced Endothelial Cell Growth Medium is used for the culture of human endothelial cells from large vessels. Defined human recombinant growth factors, including VEGF and Long R3 IGF-1, replace the crude endothelial cell growth supplement. The complete medium contains foetal calf serum, L-glutamine, heparin, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For faster expansion with a higher serum content, choose Endothelial Cell Proliferation Medium. For a formulation without VEGF, choose Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 2 % FCS (10 ml per 500 ml of basal medium).

€180.00

excl. tax and shipping

Cytion Microvascular endothelial cell growth medium, advanced, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850019

Advanced Microvascular Endothelial Cell Growth Medium

Advanced Microvascular Endothelial Cell Growth Medium is used for the culture of human microvascular endothelial cells, for example from dermis, lung or heart. Defined human recombinant growth factors replace the crude endothelial cell growth supplement. The complete medium contains foetal calf serum, L-glutamine, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For a formulation with ECGS/H and without VEGF, choose Microvascular Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€200.00

excl. tax and shipping

Chondrocyte Growth Medium

Chondrocyte Growth Medium is used for the monolayer expansion of human and bovine chondrocytes, for example before transfer into pellet or scaffold culture. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€310.00

excl. tax and shipping

Cytion Defined fibroblast growth medium-maintenance, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850143

Defined Fibroblast Maintenance Medium

Defined Fibroblast Maintenance Medium is a serum-free, fully defined medium that keeps human fibroblasts in a stable state rather than driving rapid expansion. It suits secretome, extracellular matrix and compound testing work where serum would interfere. The complete medium contains L-glutamine, human recombinant bFGF and human recombinant insulin. For the expansion of fibroblasts, choose Fibroblast Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the serum-free supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately.

€170.00

excl. tax and shipping

Endothelial Cell Growth Medium

Endothelial Cell Growth Medium is the standard medium for the culture of human endothelial cells from large vessels, such as umbilical vein, umbilical artery, saphenous vein and aorta. The complete medium contains foetal calf serum, L-glutamine, ECGS/H (endothelial cell growth supplement with heparin), EGF, bFGF and hydrocortisone. For defined recombinant growth factors including VEGF instead of ECGS/H, choose Advanced Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 2 % FCS (10 ml per 500 ml of basal medium).

€200.00

excl. tax and shipping

Endothelial Cell Proliferation Medium

Endothelial Cell Proliferation Medium is designed for the fast expansion of human endothelial cells from large vessels, such as umbilical vein, umbilical artery, saphenous vein, aorta, coronary and pulmonary artery. The complete medium contains foetal calf serum, L-glutamine, heparin, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For a lower serum content, choose Advanced Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 7 % FCS (35 ml per 500 ml of basal medium).

€170.00

excl. tax and shipping

Fibroblast Growth Medium

Fibroblast Growth Medium is used for the culture of human fibroblasts, for example from dermis or gingiva. For serum-free maintenance under defined conditions, choose Defined Fibroblast Maintenance Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€180.00

excl. tax and shipping

Cytion Mammary epithelial growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850185

Mammary Epithelial Cell Growth Medium

Mammary Epithelial Cell Growth Medium is a serum-free medium for the culture of human mammary epithelial cells. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and the individual supplement components are also available separately.

€280.00

excl. tax and shipping

Melanocyte Growth Medium

Melanocyte Growth Medium is a serum-free medium for the culture of human epidermal melanocytes. The complete medium contains L-glutamine, bovine pituitary extract (BPE), human recombinant bFGF, hydrocortisone, human recombinant insulin and PMA. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately.

€270.00

excl. tax and shipping

Microvascular Endothelial Cell Growth Medium

Microvascular Endothelial Cell Growth Medium is used for the culture of human microvascular endothelial cells, for example from dermis, lung or heart. The complete medium contains foetal calf serum, L-glutamine, ECGS/H (endothelial cell growth supplement with heparin), EGF and hydrocortisone. For defined recombinant growth factors including VEGF, choose Advanced Microvascular Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€180.00

excl. tax and shipping

Osteoblast Growth Medium

Osteoblast Growth Medium is used for the proliferation of human osteoblasts. The basal medium is formulated without calcium and magnesium to limit mineral deposition during expansion. To induce mineralisation, switch to Osteoblast Differentiation Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€300.00

excl. tax and shipping

Skeletal Muscle Cell Growth Medium

Skeletal Muscle Cell Growth Medium keeps human skeletal myoblasts proliferating and avoids premature fusion, so the cells can be expanded before differentiation. The complete medium contains foetal calf serum, L-glutamine, fetuin, EGF, bFGF, human recombinant insulin and dexamethasone. For fusion into myotubes, switch to Skeletal Muscle Cell Differentiation Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€210.00

excl. tax and shipping

Smooth Muscle Cell Growth Medium

Smooth Muscle Cell Growth Medium is used for the culture of human smooth muscle cells from vascular and non-vascular tissue, such as aorta, coronary and pulmonary artery, bronchus, trachea and bladder. The complete medium contains foetal calf serum, L-glutamine, EGF, bFGF and human recombinant insulin. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€240.00

excl. tax and shipping