B16

The B16 cell line is a widely used murine model derived from melanoma tumors in C57BL/6 mice. This line is extensively employed in research due to its ability to form melanotic tumors that closely resemble human melanoma in terms of growth characteristics and metastatic potential. The cell line exists in various subtypes, such as B16-F0, B16-F1, and B16-F10, with each subtype demonstrating varying degrees of metastatic capability; for instance, B16-F10 is highly metastatic compared to B16-F0. These variations allow researchers to select an appropriate model based on the specific requirements of their studies concerning tumor aggressiveness and metastasis.

B16 cells are instrumental in understanding the molecular and cellular mechanisms of melanoma progression and testing anti-cancer therapies. Their melanin-producing ability makes them particularly useful for studies on melanogenesis and its regulation. Furthermore, the B16 cell line serves as an essential tool for vaccine development and immunotherapy experiments, offering insights into tumor-immune system interactions and the efficacy of immunomodulatory agents. The adaptability of these cells to various in vivo and in vitro environments underscores their significance in translational and preclinical research aimed at melanoma treatment and prevention.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.