B16-F10

The B16-F10 cell line is a subline of the murine B16 melanoma cell line, derived from a spontaneous skin tumor in a mouse. These cells are characterized by their aggressive metastatic potential, particularly to the lungs, making them a valuable model for studying melanoma progression and metastasis. The B16-F10 cells exhibit high melanin content, which contributes to their pigmentation and is used as a marker in various assays to track cell proliferation and tumor growth. B16-F10 was obtained through a ten-time selective procedure using Fidler's method, enhancing its metastatic capability compared to its parent line, B16-F0, and the B16-F1 subline, which underwent a one-time selective procedure.

B16-F10 cells are widely used in cancer research due to their ability to form tumors in syngeneic C57BL/6 mice, providing a consistent and reproducible model for in vivo studies. These cells express various melanoma-associated antigens, which are crucial for investigating immune responses and developing immunotherapies. Additionally, B16-F10 cells are used to evaluate the efficacy of chemotherapeutic agents and the molecular mechanisms underlying drug resistance in melanoma. The cell line's genetic profile and behavior under different experimental conditions offer insights into the pathways involved in melanoma metastasis, aiding in the development of targeted therapeutic strategies. It is noteworthy that B16-F10's derivative, B16-BL6, exhibits even greater invasive activity, making the B16 series a comprehensive model system for studying different aspects of melanoma biology and therapy.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.