HBL-52

HBL-52 is a human cell line derived from a transitional meningioma grade I, specifically localized at the optic canal. This cell line originates from a female adult patient and exhibits epithelial-like morphology. Meningiomas are typically benign tumors that arise from the meninges, the membranous layers surrounding the brain and spinal cord. The transitional subtype represents a histological category where the tumor cells demonstrate a mixture of fibrous and meningothelial characteristics.

Recent studies have highlighted the responsiveness of HBL-52 cells to resveratrol, a naturally occurring polyphenol with significant anti-inflammatory and anticancer properties. Resveratrol has been found to inhibit proliferation in HBL-52 meningioma cells, suggesting a potential therapeutic role in managing or treating meningiomas, particularly those located in critical areas like the optic canal. This inhibition of cell proliferation highlights the utility of HBL-52 in pharmacological research and drug testing, providing a valuable model for assessing the efficacy of compounds that may influence tumor growth dynamics. Given its origin and benign nature, the HBL-52 cell line is a valuable model for studying meningioma pathogenesis, particularly in understanding the cellular behaviors and molecular mechanisms underlying the development and progression of meningiomas at unique anatomical sites like the optic canal.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.