OE19

OE19 is a human oesophageal adenocarcinoma cell line derived from the primary tumor of a patient with Barrett’s oesophagus-associated adenocarcinoma. This cell line is widely utilized in research focused on oesophageal cancers, particularly for investigating tumorigenesis in the context of Barrett’s oesophagus progression. OE19 serves as a model to study the molecular mechanisms underlying adenocarcinoma development, therapeutic responses, and resistance mechanisms in upper gastrointestinal malignancies.

OE19 cells exhibit an epithelial morphology and adhere under standard culture conditions. They are characterized by genomic alterations and molecular features typical of oesophageal adenocarcinoma, including overexpression of HER2/neu (ERBB2), a hallmark of aggressive tumor behavior and a clinically significant target for therapy. This makes OE19 particularly relevant for testing HER2-targeted therapies, such as monoclonal antibodies and tyrosine kinase inhibitors. Additionally, OE19 cells are used to explore signaling pathways critical to cancer progression, including MAPK/ERK and PI3K/AKT pathways, as well as mechanisms of immune evasion and interaction with the tumor microenvironment.

In preclinical studies, OE19 is valuable for evaluating chemotherapeutic agents, targeted therapies, and novel combinations aimed at overcoming drug resistance. The cell line is also employed in xenograft models to assess tumor growth and therapeutic efficacy in vivo. Its molecular profile and relevance to Barrett’s oesophagus-related adenocarcinoma make OE19 a significant resource for advancing the understanding and treatment of this challenging malignancy.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.