SJCRH30

SJCRH30 is a human rhabdomyosarcoma cell line derived from the bone marrow of a child with metastatic alveolar rhabdomyosarcoma. It is classified as a cancer cell line and represents the alveolar subtype of rhabdomyosarcoma, a highly aggressive soft tissue sarcoma of skeletal muscle lineage. Cytogenetic analysis has identified the characteristic chromosomal translocation t(2;13)(q35;q14), which generates the PAX3-FOXO1 fusion gene, a defining molecular feature of alveolar rhabdomyosarcoma. This translocation has been documented in primary tumors and xenografts of this subtype and is associated with tumor progression and poor clinical outcome :contentReference[oaicite:0]{index=0}. SJCRH30 cells typically grow adherently in culture and display a small round cell morphology consistent with their tumor of origin.

Functionally, SJCRH30 cells express myogenic regulatory factors, including MYOD1 and myogenin, but exhibit impaired terminal myogenic differentiation, a hallmark of rhabdomyosarcoma biology. Studies comparing rhabdomyosarcoma lines have demonstrated that although MYOD1 protein is present and capable of DNA binding, transcriptional activation of downstream differentiation programs is deficient, reflecting disruption of the normal myogenic regulatory network. The presence of the PAX3-FOXO1 fusion protein contributes to oncogenic transcriptional reprogramming, altered cell cycle regulation, and enhanced proliferative and metastatic capacity.

Comprehensive molecular profiling efforts, including large-scale genomic and proteomic analyses of cancer cell lines, have incorporated SJCRH30 as a representative alveolar rhabdomyosarcoma model. These datasets provide integrated information on copy number alterations, somatic mutations, gene expression, protein abundance, and drug sensitivity, supporting its use in studies of oncogenic signaling, therapeutic vulnerability, and biomarker discovery. Due to its well-characterized fusion status and metastatic origin, SJCRH30 is widely employed for investigating PAX3-FOXO1-driven tumor biology, epigenetic regulation, targeted therapy response, and mechanisms of resistance in high-risk pediatric soft tissue sarcoma.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.