CHO-CXCR7 Low

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The CHO-CXCR7-Low cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line expressing the CXCR7 receptor at a low level. This cell line was generated using an innovative landing pad technology, allowing for reproducible and precise integration of the CXCR7 gene at a validated genomic locus. CXCR7, also known as ACKR3, is an atypical chemokine receptor involved in various biological processes, including immune modulation and tumor biology. Unlike classical GPCRs, CXCR7 does not signal through G proteins but instead modulates cellular responses by scavenging chemokines, such as CXCL12 and CXCL11, and interacting with CXCR4 through heterodimer formation.

Overexpression of CXCR7 has been implicated in several cancers, including breast, lung, and prostate, where it is associated with enhanced tumor progression, metastasis, and poor prognosis. CXCR7 contributes to cancer progression by altering the tumor microenvironment, promoting angiogenesis, and facilitating cancer cell migration and invasion. Due to its significant role in cancer biology, CXCR7 is an important target in oncology research. The expression of CXCR7 in this cell line was confirmed using flow cytometry.

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.