HPAC

The HPAC cell line, derived from human pancreatic ductal adenocarcinoma, serves as an essential model for studying pancreatic cancer's molecular and cellular characteristics. Known for its utility in evaluating the impact of various chemotherapeutic agents and signaling pathways, HPAC cells exhibit key features typical of pancreatic cancer, including resistance mechanisms. Recent studies involving HPAC have focused on understanding drug resistance, particularly to erlotinib, a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR). Research has demonstrated that resistance to erlotinib in HPAC cells is associated with significant metabolic alterations, such as changes in phospholipid and amino acid metabolism. Specifically, increased levels of short-chain acylcarnitines and changes in glycerophospholipid profiles have been linked to an elevated metabolic state in erlotinib-resistant HPAC cells.

HPAC cells also express matrix metalloproteinases (MMPs), particularly MT1-MMP, which is crucial for their invasive behavior. The Wnt/β-catenin signaling pathway has been implicated in regulating MMP expression, contributing to the cell's migration and invasion potential. The application of compounds like matrine has been shown to inhibit HPAC cell migration by downregulating MT1-MMP through the suppression of Wnt/β-catenin signaling. These attributes highlight HPAC as a pivotal cell line for exploring therapeutic interventions aimed at mitigating pancreatic cancer's aggressive and treatment-resistant nature.

Genes expressed: keratin positive, vimentin negative, chromogranin A negative

Epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.