A875

A875 细胞系是一种人类黑色素瘤细胞系，来源于一名 40 岁女性的转移部位。A875 具有恶性黑色素瘤的典型形态和生长特征，已被广泛用于肿瘤研究，特别是黑色素瘤生物学、转移机制和治疗耐药性方面的研究。该细胞系具有侵袭性生长和转移潜能的特点，是异种移植体内肿瘤生长和转移研究的极佳模型。

A875 细胞对于研究黑色素瘤进展的分子和遗传基础尤为重要。涉及该细胞系的研究包括癌基因、肿瘤抑制基因和各种信号通路在癌症发展和进程中的作用。该细胞系已被用于新型化疗药物和靶向疗法的评估，其基因改变与人类黑色素瘤中发现的基因改变相似。此外，A875 还能引起强烈的免疫反应，因此是评估黑色素瘤免疫反应机制的关键模型，有助于开发和测试新的免疫疗法。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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