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BGM
Tissue
肾脏
Synonyms
Buffalo Green Monkey cells, BGMK, Buffalo Green Monkey Kidney cells

Documents

BGM

Cell line · Product number 302158

BGM (Buffalo Green Monkey) cells are a kidney epithelial cell line derived from the African green monkey, Cercopithecus aethiops. These cells are typicall...

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01General information

Description

BGM(水牛绿猴)细胞是一种肾上皮细胞系,来源于非洲绿猴(Cercopithecus aethiops)。这些细胞通常用于病毒学研究,因为它们对各种肠道病毒和其他病毒病原体易感,是研究病毒感染和病毒-宿主相互作用的重要工具。它们对病毒复制的高度容许性特别适用于分离和繁殖肠道病毒、轮状病毒和腺病毒等。

除用于病毒学外,BGM 细胞还可用于细胞毒性测试和疫苗生产。它们为测试新药和潜在疫苗对细胞健康和活力的影响提供了一致且受控的环境。BGM 细胞还可用于基因研究,特别是了解病毒感染和宿主反应机制中的基因表达和信号通路。BGM 细胞生长旺盛,易于在实验室环境中处理,这进一步促进了它们在生物研究中的广泛应用。

Tissue
肾脏
Applications
水传播病毒的分离
Synonyms
  • Buffalo Green Monkey cells
  • BGMK
  • Buffalo Green Monkey Kidney cells

02Classification

Organism
Vervet monkey
Sex
Male
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮样

04Identifiers

Citation
BGM(Cytion 目录号 302158)
NCBI TaxID
60710
Cellosaurus
CVCL_4125

05Handling and culture

Culture medium
EMEM(MEM Eagle),w:2 mM L-谷氨酰胺,w:2.2 g/L NaHCO3,w:EBSS(Cytion 文章编号 820100a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS 和 1% NEAA
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基,用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS,T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞,T25 烧瓶用 1-2 毫升,T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟,使其分离。孵育后,用 10 毫升培养基轻轻混合细胞使其重悬,然后用 300xg 离心 3 分钟。弃去上清液,用新鲜培养基重悬细胞,并将其转移到已装有新鲜培养基的新烧瓶中。
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

06Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染,每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基(包括 FBS)+10% DMSO 或 CM-1(Cytion 商品目录编号 800100)作为冷冻保存培养基,以获得足够的解冻后存活率,CM-1(Cytion 商品目录编号 800100)含有优化的渗透保护剂和代谢稳定剂,可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。