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DU4475
Tissue
乳房
Disease
乳腺癌
Metastatic site
皮肤
Synonyms
Du4475, DU-4475, Du-4475, DU 4475, Du 4475, Duke University 4475

Documents

DU4475

Cell line · Product number 300371

The DU4475 cell line is a human breast cancer cell line derived from a metastatic site. It is characterized by its aggressive nature and poor differentiat...

US$395.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

DU4475 细胞系是一种源自转移部位的人类乳腺癌细胞系。它具有侵袭性和分化差的特点，常用于研究癌症转移和进展的机制。该细胞系已被广泛用于探索治疗靶点和抗癌药物对高侵袭性乳腺癌类型的疗效。

在遗传学上，DU4475 表现出高度的遗传不稳定性，这是许多癌细胞的特征。这一特点使其成为研究导致癌症发展和恶化的遗传和分子事件的宝贵模型。涉及 DU4475 的研究通常侧重于调控癌细胞生长、存活和抗化疗的途径，这使它成为旨在开发更有效癌症治疗方法的肿瘤研究的重要资源。

Tissue
乳房
Disease
乳腺癌
Metastatic site
皮肤
Applications
三维细胞培养、免疫肿瘤学
Synonyms
  • Du4475
  • DU-4475
  • Du-4475
  • DU 4475
  • Du 4475
  • Duke University 4475

02Classification

Organism
Human
Sex
Female
Growth properties
Suspension
Biosafety level
1

03Characteristics

Morphology
上皮细胞
Tumourigenic
是，在裸鼠身上
Karyotype
人类平模近四倍体核型，多倍体率为 12% - 88-93<4n>xxxx, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 --20、-20、+del(7)(p11) 的侧线 --乳腺癌中典型的 1q 增益和 6q 缺失 --与已发表的核型相似

04Identifiers

Citation
DU4475（Cytion 目录号 300371）
NCBI TaxID
9606
Cellosaurus
CVCL_1183

05Expression and susceptibility

Isoenzymes
AK-1，1，ES-D，1，G6PD，B，GLO-I，2，Me-2，2，PGM1，1-2，PGM3，1
Viruses
EBV -、HBV -、HCV -、HIV-1 -、HIV-2 -、HTLV-1/2 -、MLV -、SMRV -

06Handling and culture

Culture medium
RPMI 1640，w：2.0 mM 稳定谷氨酰胺，w：2.0 g/L NaHCO3（Cytion 文章编号 820700a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 15% 热灭活 FBS
Subculturing
通过定期添加或更换培养基来维持细胞培养。初始培养时细胞密度应为5×10⁵个细胞/毫升，并保持细胞浓度在3×10⁵至1×10⁶个细胞/毫升范围内以获得最佳生长效果。
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
62 岁
Ethnicity
欧洲