Skip to content
HROC40
Tissue
降结肠，UICC IIIa
Disease
原发性腺癌，TNM 分期 T3N1M0R0L1V1，分级 G3，Lk(n) + 2，Σ Lk(n) 18

Documents

HROC40

Cell line · Product number 300822

This is one cell line of a series of tumor cell lines which have been established by PD Dr. Michael Linnebacher from Primary CRC resection specimens since 2006.

US$800.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
1

01General information

Description
该细胞系是迈克尔-林内巴赫博士自 2006 年以来从原发性 CRC 切除标本中建立的一系列肿瘤细胞系中的一个。
Tissue
降结肠，UICC IIIa
Disease
原发性腺癌，TNM 分期 T3N1M0R0L1V1，分级 G3，Lk(n) + 2，Σ Lk(n) 18

02Classification

Organism
Human
Sex
Male
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮样
Tumourigenic
是，在免疫抑制裸鼠中
Ploidy status
非整倍体
MSI status
MSS

04Identifiers

Citation
HROC40（Cytion 目录号 300822）
NCBI TaxID
9606
Cellosaurus
CVCL_1G01

05Expression and susceptibility

Protein expression
β-肌动蛋白、低骨生成素、中度 Toll 样受体 (TLR)3、中度 TLR4、低 TLR7、TLR8 -、PTEN
Antigen expression
CD326+, CD44+, CD15+, CD71+, CD73+, CD274+, CD47+, CD54+, CD95+, CD276+, CD133- , CD66acdeweak, IDO+, cFLIP+, MHC-I+, IFN-y 处理后 MHCIIweak, EpCAM+
Mutational profile
P53G266e、APCwt、K-RasG13D、mt13、N-Raswt、H-Raswt、PIK3CAwt、B-Rafwt
Viruses
不含人类致病病毒 SV40、JC/BK、HBV、HCV 和 HIV。

06Handling and culture

Culture medium
DMEM:Ham's F12（1:1），w: 3.1 g/L 葡萄糖，w: 2.5 mM L-谷氨酰胺，w: 15 mM HEPES，w: 0.5 mM 丙酮酸钠，w: 1.2 g/L NaHCO3（Cytion 文章编号 820400a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
解冻后，小心重悬细胞团。300 x g 离心 3 分钟，弃去上清液。接种到 2x 25 平方厘米的细胞培养瓶中，在培养箱中放置 48 小时。每 2-3 天更换一次用过的培养基，直到达到 80-90% 的融合度。这大约需要 10-14 天。
Seeding density
解冻后为5×10⁴个细胞/cm²，细胞旺盛增殖时为3×10⁴个细胞/cm²
Fluid renewal
每 3 至 5 天
Post-thaw recovery
快速
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
69 岁
Ethnicity
高加索人