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SNU-620
Tissue
转移性
Disease
胃腺癌
Metastatic site
腹水
Synonyms
SNU620, NCI-SNU-620

Documents

SNU-620

Cell line · Product number 305910

SNU-620 cells are a human gastric carcinoma cell line established from malignant ascites of an adult patient with poorly differentiated adenocarcinoma. Th...

US$550.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

SNU-620细胞系是从一名患有低分化腺癌的成年患者的恶性腹水中建立的人胃癌细胞系。该细胞系属于一套胃癌细胞系组合,旨在反映胃肿瘤多种多样的组织病理学和生物学特征。 体外实验显示,SNU-620细胞呈散在生长模式,细胞分布不均且对培养基质附着性有限,这反映了其源自一种纤维化程度极低、分化程度较低的肿瘤。形态学上,这些细胞主要呈圆形至椭圆形,核质比相对较低,且微绒毛发育不良,这一特征通过超微结构分析得以证实。

SNU-620细胞通过表达肿瘤相关抗原及胃恶性肿瘤特有的分子改变,为胃癌生物学研究提供了重要依据。 对相关SNU胃癌细胞系的更广泛特征分析揭示了关键致癌基因和肿瘤抑制基因(包括p53)中频繁发生的突变,这突显了其在研究胃癌遗传不稳定性和致癌信号通路方面的相关性。这些特征使SNU-620细胞成为研究胃癌肿瘤进展、转移及治疗反应的有用体外模型。

Tissue
转移性
Disease
胃腺癌
Metastatic site
腹水
Synonyms
  • SNU620
  • NCI-SNU-620

02Classification

Organism
Human
Sex
Female
Growth properties
Suspension
Biosafety level
1

03Characteristics

Doubling time
31 小时

04Identifiers

Citation
SNU-620(赛通产品编号 305910)
NCBI TaxID
9606
Cellosaurus
CVCL_5079

05Handling and culture

Culture medium
RPMI 1640,w:2.0 mM 稳定谷氨酰胺,w:2.0 g/L NaHCO3(Cytion 文章编号 820700a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
无
Seeding density
0.1 至 1 × 10⁶ /毫升
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

06Quality control and cryopreservation

Freeze medium
作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

07Donor

Donor age
59 岁
Ethnicity
韩语