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SNU-620
Tissue
轉移性
Disease
胃腺癌
Metastatic site
腹水
Synonyms
SNU620, NCI-SNU-620

Documents

SNU-620

Cell line · Product number 305910

SNU-620 cells are a human gastric carcinoma cell line established from malignant ascites of an adult patient with poorly differentiated adenocarcinoma. Th...

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Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

SNU-620 細胞是一種從一名患有低分化腺癌的成人患者之惡性腹水中建立的人類胃癌細胞系。該細胞系屬於一套胃癌細胞系組合的一部分,該組合旨在呈現胃腫瘤多樣化的組織病理學及生物學特徵。 在體外實驗中,SNU-620 細胞呈現散在生長模式,細胞分布不均且對培養基質的附著力有限,這反映出其源自一處纖維化程度極低的低分化腫瘤。 形態學上,這些細胞主要呈現圓形至橢圓形輪廓,核質比相對較低,且微絨毛發育不良,此特徵可透過超微結構分析觀察到。

SNU-620 細胞透過其表達腫瘤相關抗原及胃惡性腫瘤特有的分子改變,為胃癌生物學研究作出貢獻。 對相關 SNU 胃癌細胞系的更廣泛特徵分析顯示,關鍵致癌基因和腫瘤抑制基因(包括 p53)中存在頻繁突變,這凸顯了它們在研究胃癌的遺傳不穩定性及致癌訊號傳導路徑方面的相關性。 這些特徵使 SNU-620 細胞成為研究胃癌腫瘤進展、轉移及治療反應的有用體外模型。

Tissue
轉移性
Disease
胃腺癌
Metastatic site
腹水
Synonyms
  • SNU620
  • NCI-SNU-620

02Classification

Organism
Human
Sex
Female
Growth properties
Suspension
Biosafety level
1

03Characteristics

Doubling time
31 小時

04Identifiers

Citation
SNU-620(Cytion 型號 305910)
NCBI TaxID
9606
Cellosaurus
CVCL_5079

05Handling and culture

Culture medium
RPMI 1640,含 2.0 mM 穩定谷氨醯胺,含 2.0 g/L NaHCO₃(Cytion 產品編號 820700a)
Incubation atmosphere

37°C,5% CO₂,加濕環境。

Supplements
在培養基中加入 10% 的胎牛血清(FBS)
Dissociation reagent
無
Seeding density
0.1 至 1 × 10⁶ /ml
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

06Quality control and cryopreservation

Freeze medium
作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
Storage conditions

若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。

Shipping conditions

冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。

07Donor

Donor age
59年
Ethnicity
韓文