Cell culture media

Smooth Muscle Cell Growth Medium

Smooth Muscle Cell Growth Medium is used for the culture of human smooth muscle cells from vascular and non-vascular tissue, such as aorta, coronary and pulmonary artery, bronchus, trachea and bladder. The complete medium contains foetal calf serum, L-glutamine, EGF, bFGF and human recombinant insulin. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).