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25 products

Cytion Endothelial cell growth medium, advanced, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850006

Advanced Endothelial Cell Growth Medium

Advanced Endothelial Cell Growth Medium is used for the culture of human endothelial cells from large vessels. Defined human recombinant growth factors, including VEGF and Long R3 IGF-1, replace the crude endothelial cell growth supplement. The complete medium contains foetal calf serum, L-glutamine, heparin, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For faster expansion with a higher serum content, choose Endothelial Cell Proliferation Medium. For a formulation without VEGF, choose Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 2 % FCS (10 ml per 500 ml of basal medium).

€180.00

excl. tax and shipping

Cytion Microvascular endothelial cell growth medium, advanced, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850019

Advanced Microvascular Endothelial Cell Growth Medium

Advanced Microvascular Endothelial Cell Growth Medium is used for the culture of human microvascular endothelial cells, for example from dermis, lung or heart. Defined human recombinant growth factors replace the crude endothelial cell growth supplement. The complete medium contains foetal calf serum, L-glutamine, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For a formulation with ECGS/H and without VEGF, choose Microvascular Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€200.00

excl. tax and shipping

Cytion Chondrocyte growth medium, kit with 500 ml basal medium bottle plus supplement tubes, REF 850048

Chondrocyte Growth Medium

Chondrocyte Growth Medium is used for the monolayer expansion of human and bovine chondrocytes, for example before transfer into pellet or scaffold culture. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€310.00

excl. tax and shipping

Cytion Defined fibroblast growth medium-maintenance, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850143

Defined Fibroblast Maintenance Medium

Defined Fibroblast Maintenance Medium is a serum-free, fully defined medium that keeps human fibroblasts in a stable state rather than driving rapid expansion. It suits secretome, extracellular matrix and compound testing work where serum would interfere. The complete medium contains L-glutamine, human recombinant bFGF and human recombinant insulin. For the expansion of fibroblasts, choose Fibroblast Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the serum-free supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately.

€170.00

excl. tax and shipping

DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3

DMEM:Ham's F12 is a widely recognized and extensively utilized basal medium in cell culture for biological research. It serves as a fundamental source of nutrients for the growth of various mammalian cell lines, particularly when supplemented with Fetal Bovine Serum (FBS). This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition. DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components. To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development. DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels. Quality Control • Sterile-filtered Storage and Shelf Life • Store at +2°C to +8°C, protected from light. • Once opened, store at 4°C and use within 6–8 weeks. Shipping Conditions • Ambient temperature Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality. • Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances. • If only part of the medium is to be used, remove the required amount and warm it to room temperature before use. Composition Category Components Concentration (mg/L) Amino Acids Glycine 18.75 L-Alanine 4.45 L-Arginine HCl 147.50 L-Asparagine H₂O 7.50 L-Aspartic Acid 6.65 L-Cysteine HCl H₂O 17.56 L-Cystine 2 HCl 31.29 L-Glutamic Acid 7.35 L-Glutamine 365.00 L-Histidine HCl H₂O 31.48 L-Isoleucine 54.47 L-Leucine 59.05 L-Lysine HCl 91.25 L-Methionine 17.24 L-Phenylalanine 35.48 L-Proline 17.25 L-Serine 26.25 L-Threonine 53.45 L-Tryptophan 9.02 L-Tyrosine 2 Na 2 H2O 55.79 L-Valine 52.85 Vitamins D-Biotin 0.0035 Choline Chloride 8.98 D-Calcium Pantothenate 2.24 Folic Acid 2.66 myo-Inositol 12.60 Nicotinamide 2.02 Pyridoxine HCl 0.031 Pyridoxal HCl 2.00 Riboflavin 0.219 Thiamine HCl 2.17 Vitamin B12 0.68 Inorganic Salts CaCl2 2 H2O 154.50 CuSO4 5 H2O 0.0013 Fe(NO3)3 9 H2O 0.05 FeSO4 7 H2O 0.417 KCl 311.80 MgCl2 6 H2O 61.20 MgSO4 7 H2O 100.00 NaCl 6996.00 NaHCO3 1200.00 Na2HPO4 71.02 NaH2PO4 2 H2O 70.87 ZnSO4 7 H2O 0.432 Other Components D-Glucose 3151.00 Hypoxanthine 2.40 HEPES 3574.50 Linoleic Acid 0.042 Lipoic Acid 0.105 Phenol Red Sodium Salt 8.63 Putrescine 2 HCl 0.081 Sodium Pyruvate 55.00 Thymidine 0.365

€25.00

excl. tax and shipping

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a highly versatile and widely utilized basal medium designed to support the growth of a diverse range of mammalian cells in biological research. It serves as an ideal medium for culturing primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, as well as popular cell lines like HeLa, 293, Cos-7, and PC-12. What sets DMEM apart from other media is its unique composition. It contains an impressive fourfold increase in amino acid and vitamin concentration compared to the original Eagle's Minimal Essential Medium. Initially developed with low glucose (1 g/L) and sodium pyruvate, DMEM is frequently employed with higher glucose levels, either with or without sodium pyruvate. Notably, DMEM does not contain proteins, lipids, or growth factors, necessitating supplementation. To achieve optimal growth, a common approach is to supplement DMEM with 10% Fetal Bovine Serum (FBS). Additionally, DMEM employs a sodium bicarbonate buffer system, requiring a 5-10% CO2 environment to maintain a physiological pH. Dulbecco's Modified Eagle Medium (DMEM) is highly regarded among defined media for cell and tissue culture, catering to the growth needs of various adherent cell phenotypes. It surpasses the original Eagle's Medium, developed in the 1950s for cultivating chicken cells, through the enhanced supplementary formulation known as Dulbecco's modification. This modification significantly elevates the content of select amino acids and vitamins up to fourfold compared to the original medium. In the field of cell culture, DMEM plays a vital role as a growth medium for different cell types, including primary cells, stem cells, and transformed cells. Researchers also employ the modified version of DMEM for a wide array of research applications, such as drug discovery, tissue engineering, and the study of cell signaling pathways. Quality Control • Sterile-filtered Storage and Shelf Life • Store at +2°C to +8°C, protected from light. • Once opened, store at 4°C and use within 6–8 weeks. Shipping Conditions • Ambient temperature Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality. • Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances. • If only part of the medium is to be used, remove the required amount and warm it to room temperature before use. Composition Category Components Concentration (mg/L) Amino Acids Glycine 30.00 L-Arginine HCl 84.00 L-Cystine 2 HCl 62.57 L-Glutamine 584.00 L-Histidine HCl H2O 42.00 L-Isoleucine 105.00 L-Leucine 105.00 L-Lysine HCl 146.00 L-Methionine 30.00 L-Phenylalanine 66.00 L-Serine 42.00 L-Threonine 95.00 L-Tryptophan 16.00 L-Tyrosine 2 Na 2H2O 103.79 L-Valine 94.00 Vitamins Choline chloride 4.00 D-Calcium Pantothenate 4.00 Folic Acid 4.00 myo-Inositol 7.20 Nicotinamide 4.00 Pyridoxal HCl 4.00 Riboflavin 0.40 Thiamine HCl 4.00 Inorganic Salts CaCl2 2 H2O 265.00 Fe(NO3)3 9 H2O 0.10 KCl 400.00 MgSO4 7H2O 200.10 NaCl 6400.00 NaHCO3 3700.00 NaH2PO4 2H2O 141.73 Other Components D-Glucose 4500.00 Phenol Red Sodium Salt 15.90 Sodium Pyruvate 110.00

€25.00

excl. tax and shipping

EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS

Eagle’s Minimum Essential Medium (EMEM) is one of the most widely used basal media for the cultivation of a broad range of mammalian cells, particularly adherent cell lines. Originally developed by Harry Eagle, this classic formulation contains the essential amino acids, vitamins, and inorganic salts required to support the growth of both primary cells and established cell lines under standard culture conditions. This ready-to-use, sterile-filtered liquid formulation is supplemented with Earle’s Balanced Salt Solution (EBSS), 2 mM L-glutamine, D-glucose (1.0 g/L), and 2.2 g/L sodium bicarbonate (NaHCO3), making it suitable for use in a CO2-controlled incubator atmosphere (typically 5 % CO2). The included phenol red acts as a pH indicator, allowing convenient visual monitoring of medium condition during cell culture. Key Features • Classic Eagle’s MEM formulation with Earle’s Balanced Salt Solution (EBSS) • 2 mM L-glutamine included – ready for immediate use • 2.2 g/L sodium bicarbonate – buffered for 5 % CO2 incubation • With D-glucose (1.0 g/L) as primary carbon source • With phenol red as pH indicator • Without HEPES and without sodium pyruvate • Sterile-filtered liquid medium, ready to use • pH 7.0 – 7.6 Typical Applications EMEM supports the cultivation of a wide variety of mammalian cell lines, including HeLa, HEK 293, Vero, MRC-5, L-929, BHK-21, and many primary cells. Common applications include: • Routine maintenance and expansion of adherent cell lines • Virus propagation and vaccine production workflows • Cytotoxicity and bioassay applications • Transfection and protein expression studies • Foundational research in cell biology and molecular biology For optimal cell growth, EMEM is typically supplemented with 5–10 % fetal bovine serum (FBS) and, depending on the cell line, with non-essential amino acids (NEAA) and antibiotics such as penicillin/streptomycin. Handling & Storage Store the unopened bottle at +2 °C to +8 °C, protected from light. After opening, use under aseptic conditions. L-Glutamine in solution is subject to gradual degradation – we recommend using the medium within 4 weeks of opening for best performance, or supplementing with fresh L-glutamine prior to use if stored for longer periods. Allow the medium to warm to 37 °C before adding it to cells. Quality Manufactured under strict quality standards. Each batch is tested for sterility, pH, osmolality, and endotoxin levels to ensure consistent performance in cell culture applications. Product Specifications Specification Detail Product typeMEM Product categoryCell culture media FormatLiquid SterileYes Size500 ml L-GlutamineWith L-glutamine (2 mM) GlucoseWith glucose (1.0 g/L) Sodium bicarbonateWith NaHCO3 (2.2 g/L) HEPESWithout HEPES Sodium pyruvateWithout sodium pyruvate Phenol redWith phenol red Salt solutionEarle’s Balanced Salt Solution (EBSS) pH7.0 – 7.6 Endotoxin contentNot specified Storage+2 °C to +8 °C Formulation (Composition per Liter)

€25.00

excl. tax and shipping

Cytion Endothelial cell growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850176

Endothelial Cell Growth Medium

Endothelial Cell Growth Medium is the standard medium for the culture of human endothelial cells from large vessels, such as umbilical vein, umbilical artery, saphenous vein and aorta. The complete medium contains foetal calf serum, L-glutamine, ECGS/H (endothelial cell growth supplement with heparin), EGF, bFGF and hydrocortisone. For defined recombinant growth factors including VEGF instead of ECGS/H, choose Advanced Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 2 % FCS (10 ml per 500 ml of basal medium).

€200.00

excl. tax and shipping

Cytion Endothelial cell proliferation medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850002

Endothelial Cell Proliferation Medium

Endothelial Cell Proliferation Medium is designed for the fast expansion of human endothelial cells from large vessels, such as umbilical vein, umbilical artery, saphenous vein, aorta, coronary and pulmonary artery. The complete medium contains foetal calf serum, L-glutamine, heparin, EGF, bFGF, VEGF, Long R3 IGF-1, ascorbic acid and hydrocortisone. For a lower serum content, choose Advanced Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 7 % FCS (35 ml per 500 ml of basal medium).

€170.00

excl. tax and shipping

Cytion Fibroblast growth medium, kit with 500 ml basal medium bottle plus supplement tubes, REF 850066

Fibroblast Growth Medium

Fibroblast Growth Medium is used for the culture of human fibroblasts, for example from dermis or gingiva. For serum-free maintenance under defined conditions, choose Defined Fibroblast Maintenance Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€180.00

excl. tax and shipping

Freeze Medium CM-1 - 100 ml

Cytion’s Freeze Medium CM-1 is a state-of-the-art cryopreservation medium designed to ensure the highest level of cell viability and functionality post-thaw. This versatile medium is suitable for a broad spectrum of cell types, including both human and animal cells, making it an essential tool for diverse research applications. Formulated with a meticulously balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-1 minimizes ice crystal formation and cellular stress during the freezing process, thus preserving cellular integrity. Key features of Freeze Medium CM-1 include: • Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines. • High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes. • Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination. • Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results. • Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year. Using CM-1 for Freezing Cells To use CM-1 for freezing both adherent and suspension cells, follow these steps: • For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step. • Count the cells to ensure they are at the proper concentration. • Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium. • Transfer the resuspended cells into cryovials. • Use a slow-freezing method before transferring the cells to long-term storage. Method Description Steps ❄️ Manual Freezing A step-by-step method involving gradual temperature reduction to ensure cell viability. 1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes. 2️⃣ Transfer to a -80°C freezer for 24 hours. 3️⃣ Store cells in liquid nitrogen for long-term preservation. ❄️ Using Mr. Frosty A convenient device that allows for controlled freezing rates without electrical power. 1️⃣ Prepare cells in cryovials with freeze medium. 2️⃣ Place cryovials in Mr. Frosty container. 3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen. ❄️ Controlled-Rate Freezer A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction. 1️⃣ Program the device to gradually decrease the temperature. 2️⃣ Place prepared cells in the freezer. 3️⃣ After the freezing cycle, transfer cells to liquid nitrogen. • Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation. Ingredients • Contains FBS, DMSO, Glucose, Salts • Buffering capacity: pH = 7.2 to 7.6 Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.

€99.00

excl. tax and shipping

Freeze Medium CM-1 - 50 ml

Cytion’s Freeze Medium CM-1 is a state-of-the-art cryopreservation medium designed to ensure the highest level of cell viability and functionality post-thaw. This versatile medium is suitable for a broad spectrum of cell types, including both human and animal cells, making it an essential tool for diverse research applications. Formulated with a meticulously balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-1 minimizes ice crystal formation and cellular stress during the freezing process, thus preserving cellular integrity. Key features of Freeze Medium CM-1 include: • Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines. • High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes. • Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination. • Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results. • Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year. Using CM-1 for Freezing Cells To use CM-1 for freezing both adherent and suspension cells, follow these steps: • For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step. • Count the cells to ensure they are at the proper concentration. • Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium. • Transfer the resuspended cells into cryovials. • Use a slow-freezing method before transferring the cells to long-term storage. Method Description Steps ❄️ Manual Freezing A step-by-step method involving gradual temperature reduction to ensure cell viability. 1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes. 2️⃣ Transfer to a -80°C freezer for 24 hours. 3️⃣ Store cells in liquid nitrogen for long-term preservation. ❄️ Using Mr. Frosty A convenient device that allows for controlled freezing rates without electrical power. 1️⃣ Prepare cells in cryovials with freeze medium. 2️⃣ Place cryovials in Mr. Frosty container. 3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen. ❄️ Controlled-Rate Freezer A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction. 1️⃣ Program the device to gradually decrease the temperature. 2️⃣ Place prepared cells in the freezer. 3️⃣ After the freezing cycle, transfer cells to liquid nitrogen. • Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation. Ingredients • Contains FBS, DMSO, Glucose, Salts • Buffering capacity: pH = 7.2 to 7.6 Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.

€59.00

excl. tax and shipping

Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3

Introducing Ham's F-12K (Kaighn's) Medium, a specialized modification of Ham's F-12 medium designed to meet the unique requirements of biological research. This advanced medium offers distinct advantages, enhancing the cultivation of primary human hepatocytes, as well as rat and chicken liver cells, particularly in reduced serum conditions. Ham's F-12K (Kaighn's) Medium is carefully formulated to optimize cell culture conditions. It features an enriched composition, providing elevated levels of essential components such as amino acids and sodium pyruvate, as well as additional elements including putrescine, thymidine, hypoxanthine, and zinc. These additions enable researchers to supplement the medium with minimal serum or defined components for specific cell types, facilitating precise experimental conditions. Notably, Ham's F-12K (Kaighn's) Medium does not contain proteins or growth factors. Consequently, supplementation with growth factors and Fetal Bovine Serum (FBS) is often necessary, allowing researchers to tailor the medium to the requirements of their specific cell lines. For optimal performance, the concentration of FBS must be carefully optimized for each cell line, ensuring optimal growth and functionality. To maintain physiological pH, Ham's F-12K (Kaighn's) Medium employs a sodium bicarbonate buffer system (2.5 g/L), necessitating a controlled 5-10% CO2 environment during cultivation. This ensures the medium's pH remains within the ideal range for cell growth and viability. Quality control • pH = 7.2 +/- 0.02 at 20-25°C. • Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product. • Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. • Shelf life for any medium but the basic medium is 8 weeks from the date of manufacture. Composition Components mg/L Inorganic Salts Calcium chloride x 2H2O 135,24 Copper(II) sulfate x 5H2O 0,00 Iron (II) sulfate x 7H2O 0,83 Magnesium chloride x 6H2O 105,72 Magnesium sulfate x 7H2O 394,49 Potassium chloride 283,29 Potassium dihydrogen phosphate 58,52 Sodium chloride 7597,20 di-Sodium hydrogen phosphate anhydrous 115,02 Zinc sulfate x 7H2O 0,14 Other Components D(+)-Glucose anhydrous 1260,00 Hypoxanthine 4,08 DL-α-Lipoic acid 0,21 Phenol red 3,00 Putrescine x 2HCl 0,32 Sodium pyruvate 220,00 NaHCO3 2500,00 Thymidine 0,73 Amino Acids L-Alanine 17,82 L-Arginine x HCl 421,40 L-Asparagine x H2O 30,02 L-Aspartic acid 26,62 L-Cysteine x HCl x H2O 70,24 L-Glutamine 292,20 L-Glutamic acid 29,42 Glycine 15,01 L-Histidine x HCl x H2O 41,92 L-Isoleucine 7,87 L-Leucine 26,24 L-Lysine x HCl 73,04 L-Methionine 8,95 L-Phenylalanine 9,91 L-Proline 69,06 L-Serine 21,02 L-Threonine 23,82 L-Tryptophan 4,08 L-Tyrosine 10,87 L-Valine 23,42 Vitamins D(+)-Biotin 0,07 D-Calcium pantothenate 0,48 Choline chloride 13,96 Folic acid 1,32 myo-Inositol 18,02 Nicotinamide 0,04 Pyridoxine x HCl 0,06 Riboflavin 0,04 Thiamine x HCl 0,34 Vitamin B12 1,36

€108.00

excl. tax and shipping

Ham's F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3

Ham's F-12 Medium, also known as Ham's F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA). One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations. Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility. Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles. Quality control • pH = 7.2 +/- 0.02 at 20-25°C. • Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. • Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. • Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. Composition Components mg/L Inorganic Salts Calcium chloride x 2H2O 44,00 Copper(II) sulfate x 5H2O 0,00 Iron (II) sulfate x 7H2O 0,83 Magnesium chloride x 6H2O 122,00 Potassium chloride 223,65 Sodium chloride 7599,00 di-Sodium hydrogen phosphate anhydrous 142,04 Zinc sulfate x 7H2O 0,86 Other Components D(+)-Glucose anhydrous 1801,60 Hypoxanthine 4,08 Linoleic acid 0,08 DL-α-Lipoic acid 0,21 Phenol red 1,20 Putrescine x 2HCl 0,16 Sodium pyruvate 110,00 Thymidine 0,73 NaHCO3 1176,00 Amino Acids L-Alanine 8,91 L-Arginine x HCl 210,70 L-Asparagine x H2O 15,01 L-Aspartic acid 13,31 L-Cysteine x HCl x H2O 35,12 L-Alanyl-L-Glutamine 217,30 L-Glutamic acid 14,71 Glycine 7,51 L-Histidine x HCl x H2O 20,96 L-Isoleucine 3,94 L-Leucine 13,12 L-Lysine x HCl 36,54 L-Methionine 4,48 L-Phenylalanine 4,96 L-Proline 34,53 L-Serine 10,51 L-Threonine 11,91 L-Tryptophan 2,04 L-Tyrosine 5,44 L-Valine 11,71 Vitamins D(+)-Biotin 0,01 D-Calcium pantothenate 0,24 Choline chloride 13,96 Folic acid 1,32 myo-Inositol 18,02 Nicotinamide 0,04 Pyridoxine x HCl 0,06 Riboflavin 0,04 Thiamine x HCl 0,34 Vitamin B12 1,36

€25.00

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IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3

Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH. IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium. IMDM vs. DMEM IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM. IMDM vs. RPMI IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM. Quality control • pH = 7.2 +/- 0.02 at 20-25°C. • Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. • Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. • Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. Composition Components mg/L Inorganic Salts Calcium chloride x 2 H2O 219,00 Potassium chloride 330,00 Potassium nitrate 0.076 Magnesium sulfate anhydrous 97,73 Sodium chloride 4,505.00 Sodium dihydrogen phosphate anhydrous 109,00 Sodium selenite 0,02 Other Components D(+)-Glucose anhydrous 4,500.00 HEPES 5,958.00 Sodium pyruvate 110,00 Phenol red 15,00 Amino Acids L-Alanine 25,00 L-Arginine x HCl 84,00 L-Asparagine x H2O 25,00 L-Aspartic acid 30,00 L-Cystine x 2HCl 91,24 L-Glutamine 584,00 L-Glutamic acid 75,00 Glycine 30,00 L-Histidine x HCl x H2O 42,00 L-Isoleucine 104,80 L-Leucine 104,80 L-Lysine x HCl 146,20 L-Methionine 30,00 L-Phenylalanine 66,00 L-Proline 40,00 L-Serine 42,00 L-Threonine 95,20 L-Tryptophan 16,00 L-Tyrosine x 2Na 104,20 L-Valine 93,60 Vitamins D(+)-Biotin 0.013 D-Calcium pantothenate 4,00 Choline chloride 4,00 Folic acid 4,00 myo-Inositol 7,20

€25.00

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Cytion Mammary epithelial growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850185

Mammary Epithelial Cell Growth Medium

Mammary Epithelial Cell Growth Medium is a serum-free medium for the culture of human mammary epithelial cells. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and the individual supplement components are also available separately.

€280.00

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McCoys 5A medium (modified), w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3

McCoy's 5A Medium is a highly recommended and specialized medium designed to facilitate the growth and replication of viruses in primary cell cultures. It has gained significant recognition for its exceptional performance in various biological research applications. One prominent application of McCoy's 5A Medium is its utilization in the culturing of human colon carcinoma cell lines. Specifically, it has been employed in the study of the leucine-rich repeat-containing G-protein-coupled receptor (LGR5) and its role in the metastasis of colon cancer. This medium has been effectively employed in the cultivation of several colon carcinoma cell lines, including HCT116, RKO, FET, CBS, HCT116b, and TENN, enabling researchers to delve deeper into the mechanisms underlying colon cancer metastasis. In addition to its application in cancer research, McCoy's 5A Medium has proven to be indispensable in the study of osteoblasts. Researchers investigating the ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media have utilized this medium to culture osteoblasts. This application has facilitated a better understanding of the interactions between osteoblasts and calcium-deficient hydroxyapatite, contributing to advancements in the field of bone research. McCoy's 5A Medium was meticulously formulated by modifying the amino acids found in Basal Medium Eagle to provide optimal support for liver tumor cells. This enriched formulation enables its suitability for a diverse range of established cell lines, as well as primary cells, further enhancing its versatility and applicability in various research settings. McCoy's 5A Medium extends its biochemical and physiological effects beyond liver tumor cells. It has been successfully employed to support growth in primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenal, lung, spleen, rat embryo, and other cell types. This wide range of applications attests to the broad utility of McCoy's 5A Medium in supporting the growth and maintenance of various cell types for comprehensive biological research. Formulation This McCoy's 5A medium (modified) contains 3.0 g/L of Glucose, stable Glutamine, 2.0 mM of Sodium pyruvate, and 2.2 g/L of NaHCO3. Quality control • pH = 7.2 +/- 0.02 at 20-25°C. • Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37°C minimize the quality of the product. • Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances). • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. • Shelf life for any medium except for the basic medium is 6 to 8 weeks from the date of opening. Composition Components mg/L Inorganic SaltsCalcium chloride x 2H2O132.00 Magnesium sulfate97.67 Potassium chloride400.00 Sodium chloride6,460.00 Disodium hydrogen phosphate (anhydrous)504.00 Other ComponentsD(+)-Glucose (anhydrous)3,000.00 Glutathione (reduced)0.50 Meat Peptone600.00 Phenol red sodium salt11.00 Amino AcidsL-Alanine13.36 L-Arginine x HCl42.14 L-Asparagine x H2O45.03 L-Aspartic acid19.97 L-Cysteine x HCl x H2O31.75 L-Glutamine (stable)219.15 L-Glutamic acid22.07 Glycine7.51 L-Histidine x HCl x H2O20.96 L-Hydroxyproline19.67 L-Isoleucine39.36 L-Leucine39.36 L-Lysine x HCl36.54 L-Methionine14.92 L-Phenylalanine16.52 L-Proline17.27 L-Serine26.28 L-Threonine17.87 L-Tryptophan3.06 L-Tyrosine disodium salt26.10 L-Valine17.57 Vitaminsp-Aminobenzoic acid1.00 Ascorbic acid0.56 D(+)-Biotin0.20 D-Calcium pantothenate0.20 Choline chloride5.00 Folic acid10.00 myo-Inositol36.00 Nicotinamide0.50 Nicotinic acid0.50 Pyridoxal HCl0.50 Pyridoxine HCl0.50 Riboflavin0.20 Thiamine HCl0.20 Vitamin B122.00

€30.00

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Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS

Medium 199 is a versatile and widely used cell culture medium specially formulated for the cultivation of primary explants. This comprehensive medium combines essential vitamins, amino acids, and other factors to provide a fully defined nutrient source for a variety of cell types. It is particularly suitable for non-transformed cells, making it an invaluable tool for biological research. Medium 199 offers a range of applications in the field. It can effectively maintain the cumulus-oocyte complex (COC) and support the in vitro maturation of oocytes. Additionally, it is employed in the rinsing of aspiration lines during ovum collection from German Holstein cows. Moreover, Medium 199 serves as an excellent medium for the culture of cardiac endothelial cells derived from rats. These applications demonstrate the versatility and adaptability of Medium 199 to various experimental needs. History The development of Medium 199 in the 1950s marked a significant advancement in tissue culture media. Prior to its introduction, many culture media relied on animal-derived products and tissue extracts. However, Morgan and colleagues revolutionized the field by formulating a completely defined nutritional source for cell cultures. Through their experiments involving different combinations of vitamins, amino acids, and other factors, they discovered the exceptional growth-promoting properties of Medium 199. Quality control • pH = 7.2 +/- 0.02 at 20-25°C. • Each lot has been tested for sterility and absence of mycoplasma and bacteria. Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. • Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. • Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. Composition Components mg/L Inorganic Salts Calcium chloride x 2H2O 264,92 Iron (III) nitrate x 9H2O 0,72 Magnesium sulfate 97,67 Potassium chloride 400,00 Sodium acetat x 3H2O 82,95 Sodium chloride 6,800.00 Sodium dihydrogen phosphate x H2O 140,00 Other Components Adenine sulfate 10,00 AMP 0,20 ATP 1,00 Cholesterol 0,20 2‘-Deoxyribose 0,50 D(+)-Glucose anhydrous 1,000.00 Glutathione (red.) 0,05 Guanine x HCl 0,30 Hypoxanthine 0,30 Phenol red 10,00 D-Ribose 0,50 Thymine 0,30 Tween 80 4,90 Uracil 0,30 Xanthine 0,30 NaHCO3 2,200.00 Amino Acids L-Alanine 25,00 L-Arginine x HCl 70,00 L-Aspartic acid 30,00 L-Cysteine x HCl x H2O 0,10 L-Cystine 20,00 L-Glutamine stable 149,00 L-Glutamic acid 67,00 Glycine 50,00 L-Histidine x HCl x H2O 21,88 L-Hydroxyproline 10,00 L-Isoleucine 20,00 L-Leucine 60,00 L-Lysine x HCl 70,00 L-Methionine 15,00 L-Phenylalanine 25,00 L-Proline 40,00 L-Serine 25,00 L-Threonine 30,00 L-Tryptophan 10,00 L-Tyrosine 40,00 L-Valine 25,00 Vitamins 4-Amino benzoic acid 0,05 Ascorbic acid 0,05 D(+)-Biotin 0,01 Calciferol 0,10 D-Calcium pantothenate 0,01 Choline chloride 0,50 Folic acid 0,01 myo-Inositol 0,05 Menadione 0,01 Nicotinic acid 0.025 Nicotinamide 0.025 Pyridoxal x HCl 0.025 Pyridoxol x HCl 0.025 Riboflavin 0,01 DL-α-Tocopherol phosphate disodium salt 0,01 Thiamine x HCl 0,01 Vitamine A acetate 0,14

€40.00

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Cytion Melanocyte growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850155

Melanocyte Growth Medium

Melanocyte Growth Medium is a serum-free medium for the culture of human epidermal melanocytes. The complete medium contains L-glutamine, bovine pituitary extract (BPE), human recombinant bFGF, hydrocortisone, human recombinant insulin and PMA. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately.

€270.00

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Cytion Microvascular endothelial cell growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850013

Microvascular Endothelial Cell Growth Medium

Microvascular Endothelial Cell Growth Medium is used for the culture of human microvascular endothelial cells, for example from dermis, lung or heart. The complete medium contains foetal calf serum, L-glutamine, ECGS/H (endothelial cell growth supplement with heparin), EGF and hydrocortisone. For defined recombinant growth factors including VEGF, choose Advanced Microvascular Endothelial Cell Growth Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. The basal medium is also available phenol red-free in the same formats, which avoids interference with fluorescence and colorimetric readouts as well as the weak oestrogen-like activity of phenol red. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€180.00

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NCI-H295R Cell Growth Medium

Discover the advanced NCI-H295R Cell Growth Medium, an expertly crafted nutrient solution specifically designed to foster the growth and maintenance of mammalian cell lines, including the specialized NCI-H295R cells. This medium stands out with its unique blend of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Nutrient Mixture F-12) in a precise 1:1 ratio, enriched with essential supplements to meet the intricate needs of cell culture. Enhanced Formulation for Superior Cell Culture Performance • Supplemented Baseline: The basic medium is already rich, combining DMEM's high amino acid and vitamin concentrations with the diverse nutrients of Ham's F-12. To this potent mix, we've added 5% Fetal Bovine Serum (FBS), providing crucial growth factors, hormones, and proteins. • Tailored Additives: Beyond FBS, this medium is fortified with critical supplements including Insulin, Transferrin, Bovine Serum Albumin, Selenious Acid, and Lineloic Acid. These additions cater to specific cellular requirements, promoting enhanced cell growth, metabolic function, and overall health. • L-Glutamine Boost: The presence of L-glutamine further amplifies the medium's efficacy, supporting essential metabolic processes and encouraging robust cell proliferation. Optimized for Cell Culture • pH Controlled Environment: Incorporating a bicarbonate buffer system and phenol red indicator, the medium ensures a stable pH environment. It is optimized for a 5% CO2 atmosphere, allowing for precise pH monitoring and adjustment. • Quality and Purity: Each lot of basic medium undergoes rigorous testing for sterility, mycoplasma, and bacteria, ensuring the medium's integrity. With a pH maintained medium, you can trust in the consistent quality and performance of this medium. Handling and Storage Maintain the medium's quality by storing it refrigerated at +2°C to +8°C, away from light. Avoid extremes, such as freezing or overheating, to preserve its efficacy. For partial use, gently warm the needed quantity at room temperature, ensuring the medium is brought to the appropriate condition without compromising its components. Shelf life With an 8-week shelf life from the manufacturing date, the NCI-H295R Cell Growth Medium is a reliable choice for your cell culture needs, ensuring the vitality and productivity of your research.

€150.00

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Cytion Osteoblast growth medium, kit with 500 ml basal medium bottle plus supplement tubes, REF 850057

Osteoblast Growth Medium

Osteoblast Growth Medium is used for the proliferation of human osteoblasts. The basal medium is formulated without calcium and magnesium to limit mineral deposition during expansion. To induce mineralisation, switch to Osteoblast Differentiation Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 10 % FCS (50 ml per 500 ml of basal medium).

€300.00

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RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3

RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI). Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth. The buffering system of RPMI 1640 Medium relies on sodium bicarbonate and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others. Quality Control • Sterile-filtered Storage and Shelf Life • Store at +2°C to +8°C, protected from light. • Once opened, store at 4°C and use within 6–8 weeks. Shipping Conditions • Ambient temperature Maintenance • Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality. • Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances. • If only part of the medium is to be used, remove the required amount and warm it to room temperature before use. Composition Category Components Concentration (mg/L) Amino Acids Glycine 10.00 L-Alanyl-L-Glutamine 434.40 L-Arginine 200.00 L-Asparagine H2O 56.82 L-Aspartic Acid 20.00 L-Cystine 2HCl 65.20 L-Glutamic Acid 20.00 L-Histidine HCl H2O 20.27 L-Hydroxy-L-Proline 20.00 L-Isoleucine 50.00 L-Leucine 50.00 L-Lysine HCl 40.00 L-Methionine 15.00 L-Phenylalanine 15.00 L-Proline 20.00 L-Serine 30.00 L-Threonine 20.00 L-Tryptophan 5.00 L-Tyrosine 2Na 2H2O 28.83 L-Valine 20.00 Vitamins p-Amino Benzoic Acid 1.00 D-Biotin 0.20 Choline Chloride 3.00 D-Calcium Pantothenate 0.25 Folic Acid 1.00 myo-Inositol 35.00 Nicotinamide 1.00 Pyridoxine HCl 1.00 Riboflavin 0.20 Thiamine HCl 1.00 Vitamin B12 0.005 Inorganic Salts Ca(NO3)2 4H2O 100.00 KCl 400.00 MgSO4 7H2O 100.00 NaCl 6000.00 NaHCO3 2000.00 Na2HPO4 800.00 Other Components D-Glucose 2000.00 L-Glutathione Reduced 1.00 Phenol Red Sodium Salt 5.30

€25.00

excl. tax and shipping

Cytion Skeletal muscle cell growth medium, kit with 500 ml basal medium bottle plus supplement tubes and vials, REF 850082

Skeletal Muscle Cell Growth Medium

Skeletal Muscle Cell Growth Medium keeps human skeletal myoblasts proliferating and avoids premature fusion, so the cells can be expanded before differentiation. The complete medium contains foetal calf serum, L-glutamine, fetuin, EGF, bFGF, human recombinant insulin and dexamethasone. For fusion into myotubes, switch to Skeletal Muscle Cell Differentiation Medium. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and both supplement formats are also available separately. Once supplemented, the complete medium contains 5 % FCS (25 ml per 500 ml of basal medium).

€210.00

excl. tax and shipping