ID8-Luc

ID8-Luc is a bioluminescent derivative of the murine ID8 ovarian surface epithelial cell line, engineered to stably express a firefly luciferase reporter gene. The parental ID8 cell line was derived from normal C57BL/6 mouse ovarian surface epithelial cells through serial in vitro passaging, during which the cells acquired a transformed, tumorigenic phenotype. ID8 cells grow as a peritoneal carcinomatosis-like model when injected intraperitoneally into syngeneic C57BL/6 mice, producing ascites and disseminated peritoneal tumors that closely recapitulate features of advanced human high-grade serous ovarian cancer. The model is widely used for studying ovarian tumor-immune interactions, tumor microenvironment biology, and immunotherapy approaches in an immunocompetent host.

The stable luciferase integration in ID8-Luc enables sensitive, noninvasive bioluminescence imaging (BLI) of tumor burden in living animals following administration of the luciferin substrate. The emitted signal correlates with viable tumor cell number, supporting longitudinal monitoring of peritoneal tumor dissemination, ascites development, and therapeutic response without repeated invasive procedures. This makes ID8-Luc especially valuable for preclinical studies evaluating immune checkpoint inhibitors, adoptive cell therapies, cancer vaccines, and novel targeted agents in a fully immunocompetent syngeneic model of ovarian cancer.

ID8-Luc retains the established tumorigenic and immunological properties of the parental ID8 line, including its intraperitoneal growth pattern and compatibility with C57BL/6 syngeneic hosts. The luciferase modification enhances experimental sensitivity and enables real-time assessment of disease progression and treatment efficacy. Researchers should validate luciferase activity, growth kinetics, and immunological phenotype under their specific experimental conditions prior to use in quantitative imaging or immunotherapy studies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.