- ティッシュ
- Lung (bronchus)
- 病気
- Normal bronchial smooth muscle (non-tumorigenic; primary cells)
- 転移部位
- Not applicable (normal primary cells, not a tumor sample)
- 同義語
- HBSMC, Human bronchial smooth muscle cells, provitro 1210632
ヒト気管支平滑筋細胞（HBSMC）
Primary cell · 製品番号 300708
喘息研究用の気道平滑筋
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- ヒト気管支平滑筋細胞（HBSMC）Primary cell€1,515.00
01概要
- 説明
Primary Human Bronchial Smooth Muscle Cells (HBSMC) are isolated from the bronchi. They are used to study airway remodeling, contractility and inflammatory signaling in asthma.
Airway smooth muscle cells are contractile and also immunomodulatory: in response to IL-13 they proliferate, migrate and produce periostin, and they release IL-6 and IL-8 upon immune stimulation. These responses link inflammation to the structural airway remodeling seen in asthma.
- ティッシュ
- Lung (bronchus)
- 病気
- Normal bronchial smooth muscle (non-tumorigenic; primary cells)
- 転移部位
- Not applicable (normal primary cells, not a tumor sample)
- 用途
- Asthma: chemokine receptors, wound repair and calcium signaling (asthmatic and healthy donors); IL-13-induced periostin production in Th2-driven airway inflammation; IL-13-driven proliferation and migration, store-operated calcium entry (Orai1); Immunoglobulin A-induced IL-6/IL-8 release and phenotype switching; Airway remodeling and smooth muscle dysfunction as a therapeutic target
- 同義語
- HBSMC
- Human bronchial smooth muscle cells
- provitro 1210632
02寄付者
- ドナーの年齢
- Lot-specific (see CoA)
- 民族
- Lot-specific (see CoA)
03分類
- 生物
- 人間
- セックス
- ロット別
- 成長特性
- アドヘレント
- GMOの有無
- 非遺伝子組み換え
- GMOのステータスに関する詳細
- No genetic modification; primary human cells. Handle as potentially biohazardous material from human origin despite negative viral testing (HIV-1, HBV, HCV).
- バイオセーフティレベル
- 1
04特徴
- 形態学
- Spindle-shaped (smooth muscle)
- 細胞の種類
- Primary smooth muscle cells
- 腫瘍形成性
- No
- 倍増時間
- approx. 36 to 48 hours (under standard culture conditions)
05取り扱いと飼育
- 培地
- Smooth Muscle Cell Growth Medium kit (Cytion article number 850075); Smooth Muscle Cell Growth Medium mix (Cytion article number 850074)
- 培養雰囲気
37°C, 5% CO2, saturated humidity.
- サプリメント
- Smooth Muscle Cell Growth Supplement (ScienceCell catalog #1152) and 2% FBS included in SMCM. Alternatively, DMEM supplemented with 10% FBS, 2 mM L-glutamine, and 0.12 IU/mL insulin has been used in published studies.
- 解離試薬
- Subculture reagents as recommended on the certificate of analysis
- 継代培養
Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.
- 分割比率
- 1 to 3
- 播種密度
- More than 4,000 cells/cm²
- 流体の更新
- 24 hours after seeding, then every 48 to 72 hours
- 解凍後の回復
Post-thaw viability ≥ 70 % viable cells. More than 80 % confluence within the expected recovery period.
- 解凍と培養
Thaw the cryovial quickly in a 37 °C water bath, swirling gently. Just before the last ice has melted, move the vial to a laminar flow hood and decontaminate its outer surface.
Transfer the cell suspension into a 15 ml centrifuge tube, add 10 ml of Smooth Muscle Cell Growth Medium and centrifuge at 250 × g for 5 minutes.
Remove the supernatant and resuspend the cell pellet in 10 ml of Smooth Muscle Cell Growth Medium. Check cell number and viability.
Divide the suspension between two 25 cm² culture flasks (5 ml each). Using two flasks reduces the risk of losing all cells if a passage fails.
Incubate at 37 °C, 5 % CO₂ and saturated humidity, with the screw caps opened by half a turn for gas exchange.
Change the medium after 24 hours to remove unattached cells, then every 48 to 72 hours, using pre-warmed medium only.
Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.
Recommended seeding density: more than 4,000 cells/cm².
06品質管理と凍結保存
- 無菌性
Mycoplasma contamination is excluded by PCR.
To ensure there is no bacterial, fungal or yeast contamination, cultures are subjected to visual inspection.
- 凍結培地
- As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
- 保管条件
For long-term preservation, store vials in liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable for up to one week only.
- 配送条件
Cryopreserved primary cells are shipped on dry ice. On receipt, check the cryovial for damage and transfer it without delay to appropriate storage.
07発現と感受性
- 表面抗原
- α-smooth muscle actin (α-SMA) +; Desmin +
- タンパク質の発現
- Smooth muscle α-actin; Desmin; Smooth muscle myosin heavy chain; Calponin; SM22; h-Caldesmon
- ウイルス
- Confirmed negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi (ScienceCell QC testing)
08識別子
- 引用
- Human bronchial smooth muscle cells (HBSMC) (Cytion catalog number 300708)
- NCBI 納税者番号
- 9606
09法務・安全
- 安全上の注意事項
- When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Primary cells are of human origin, and no known test procedure can ensure the complete absence of infectious agents. Handle all products as potentially infectious.
- 保証
At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.
For full details, please refer to our Warranty Statement.
- 物質移転契約書の締結が必要
- はい