ヒト尿路上皮平滑筋細胞（HUSMC）

01説明

02概要

説明

Primary Human Bladder Smooth Muscle Cells (HBdSMC) are isolated from the smooth muscle layer of the urinary tract. They are used to study the response of the bladder wall to mechanical stretch and as a building block for engineered bladder tissue.

HBdSMC respond to cyclic stretch and hydrostatic pressure that mimic bladder filling with increased proliferation and altered contractility, involving muscarinic receptor signaling. This makes them a model for detrusor remodeling in bladder outlet obstruction and for conditioning cells in bladder tissue engineering.

ティッシュ

Bladder

病気

Normal bladder smooth muscle / detrusor (primary cells; non-tumorigenic)

転移部位

Not applicable (normal, non-tumorigenic primary cell)

用途

Cyclic stretch of the bladder wall, muscarinic receptors and proliferation; Hydrostatic pressure in bladder outlet obstruction: proliferation and contractility; Hypoxia and oxidative stress in bladder ischemia; Rac1 signaling as a target in overactive bladder; Bladder tissue engineering with urothelial and smooth muscle cells

同義語

• HBdSMC
• HUSMC
• Human urothelial smooth muscle cells
• provitro 1210631

03寄付者

ドナーの年齢

Lot-specific (see CoA)

民族

Lot-specific (see CoA)

04分類

生物

人間

セックス

ロット別

成長特性

アドヘレント

GMOの有無

非遺伝子組み換え

GMOのステータスに関する詳細

No genetic modification; primary human bladder smooth muscle cells. Not transformed or immortalized. Confirmed negative for mycoplasma, bacteria, yeast, HIV-1, HBV, HCV.

バイオセーフティレベル

1

05特徴

形態学

Spindle-shaped (smooth muscle)

細胞の種類

Primary smooth muscle cells

腫瘍形成性

No

倍増時間

approx. 18 to 36 hours (lot-specific; AcceGen equivalent: ~13 population doublings guaranteed)

06取り扱いと飼育

培地

Smooth Muscle Cell Growth Medium kit (Cytion article number 850075); Smooth Muscle Cell Growth Medium mix (Cytion article number 850074)

培養雰囲気

37°C, 5% CO₂, saturated humidity.

サプリメント

Supplement per AcceGen medium kit instructions. Standard SMC growth medium contains FBS (5–10%), EGF, FGF, and insulin as major components. Refer to product-specific CoA.

解離試薬

Subculture reagents as recommended on the certificate of analysis

継代培養

Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

分割比率

1 to 3

播種密度

More than 4,000 cells/cm²

流体の更新

24 hours after seeding, then every 48 to 72 hours

解凍後の回復

Post-thaw viability ≥ 70 % viable cells. More than 80 % confluence within the expected recovery period.

解凍と培養

1. Thaw the cryovial quickly in a 37 °C water bath, swirling gently. Just before the last ice has melted, move the vial to a laminar flow hood and decontaminate its outer surface.

2. Transfer the cell suspension into a 15 ml centrifuge tube, add 10 ml of Smooth Muscle Cell Growth Medium and centrifuge at 250 × g for 5 minutes.

3. Remove the supernatant and resuspend the cell pellet in 10 ml of Smooth Muscle Cell Growth Medium. Check cell number and viability.

4. Divide the suspension between two 25 cm² culture flasks (5 ml each). Using two flasks reduces the risk of losing all cells if a passage fails.

5. Incubate at 37 °C, 5 % CO₂ and saturated humidity, with the screw caps opened by half a turn for gas exchange.

6. Change the medium after 24 hours to remove unattached cells, then every 48 to 72 hours, using pre-warmed medium only.

7. Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

8. Recommended seeding density: more than 4,000 cells/cm².

07品質管理と凍結保存

無菌性

Mycoplasma contamination is excluded by PCR.

To ensure there is no bacterial, fungal or yeast contamination, cultures are subjected to visual inspection.

凍結培地

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

保管条件

For long-term preservation, store vials in liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable for up to one week only.

配送条件

Cryopreserved primary cells are shipped on dry ice. On receipt, check the cryovial for damage and transfer it without delay to appropriate storage.

08発現と感受性

表面抗原

α-smooth muscle actin (α-SMA) +; Desmin +; Calponin (CNN1) +; SM-MHC (MYH11) + (differentiated); h-caldesmon + (differentiated); γ-smooth muscle actin + (differentiated); Connexin 43 +

発現している受容体

Muscarinic acetylcholine receptor M2 (CHRM2) +; Muscarinic receptor M3 (CHRM3) +; PDGFR-β (CD140b); Adrenergic receptors (β1, β3)

ウイルス

Free of HIV-1, HBV, HCV (confirmed negative; AcceGen ABC-TC3862 QC)

09識別子

引用

Human urothelial smooth muscle cells (HUSMC) (Cytion catalog number 300707)

NCBI 納税者番号

9606

セロサウルス

Not assigned

10法務・安全

安全上の注意事項

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Primary cells are of human origin, and no known test procedure can ensure the complete absence of infectious agents. Handle all products as potentially infectious.

保証

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

物質移転契約書の締結が必要

はい

11商品詳細

構成表